Description

4-Thiouridine is a modified nucleoside where the oxygen atom at the 4-position of the uracil base is replaced by a sulfur atom. This structural modification is critical for its role as a versatile biochemical tool and a key building block in nucleic acid research. It is primarily utilized in the life sciences sector for studying RNA structure, function, and for the development of novel therapeutic and diagnostic agents. This high-purity compound is essential for researchers and manufacturers requiring reliable, well-characterized materials for advanced applications.

Application

• RNA Structure and Function Studies: Used as a site-specific probe for investigating RNA folding, dynamics, and protein-RNA interactions via its unique photochemical and spectroscopic properties.
• Crosslinking Agent: Serves as a photoactivatable crosslinker in nucleic acids to map biomolecular interactions and stabilize complex structures.
• Therapeutic Oligonucleotide Synthesis: Acts as a modified monomer in the synthesis of antisense oligonucleotides, siRNAs, and aptamers to enhance stability and target specificity.
• Biochemical Labeling: Employed for the selective labeling and detection of RNA molecules in complex biological samples.
• Enzymology Research: Used as a substrate or inhibitor to study the mechanism and specificity of nucleoside/nucleotide-processing enzymes.
• Diagnostic Probe Development: Incorporated into nucleic acid probes for sensitive detection systems, leveraging its distinct physical properties.

Basic Information

Item	Details
Product Name	4-Thiouridine
CAS No.	13957-31-8
Molecular Formula	C9H12N2O5S
Molecular Weight	260.27 g/mol
Synonyms	4-Thio-uridine; 4-Sulfur-uridine; 4-Thiouridylic acid (nucleoside); 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-4-sulfanylpyrimidin-2-one; s4U; 4-thio-U; 4-thioUrd
EINECS	Contact for details

Quality Control

Our 4-Thiouridine is manufactured under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom purity grades, including >98% and >99%, are available to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.