Description

Hellebrin is a potent cardiac glycoside and steroidal saponin, a key bioactive compound derived from plants of the *Helleborus* genus. Its primary value lies in its significant pharmacological activity, particularly as a digitalis-like agent affecting heart muscle contractility. This makes it a critical reference standard and research chemical for the pharmaceutical and biotechnology sectors. Professionals in medicinal chemistry, natural product research, and toxicology rely on high-purity Hellebrin for analytical testing, bioactivity studies, and the development of novel therapeutic agents.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the identification, purity assessment, and quantification of cardiac glycosides in quality control laboratories.
• Medicinal Chemistry Research: Serves as a lead compound or biochemical tool for studying sodium-potassium ATPase (Na+/K+-ATPase) inhibition and its effects on cardiac function.
• Natural Product Isolation & Analysis: Employed as a benchmark in the extraction, purification, and chromatographic analysis (HPLC, TLC) of bioactive compounds from plant sources.
• Pharmacological & Toxicological Studies: Utilized in *in vitro* and *in vivo* research to investigate cardiotonic effects, therapeutic windows, and potential toxicity profiles.
• Biochemical Assay Development: Acts as a specific inhibitor in enzymatic assays to study ion transport mechanisms and signal transduction pathways.
• Academic & Institutional Research: Supports fundamental research in botany, pharmacology, and cell biology within universities and government research institutes.

Basic Information

Product Name	Hellebrin
CAS No.	13289-18-4
Molecular Formula	C37H56O18
Molecular Weight	788.83 g/mol
Synonyms	Corelborin; Hellebrigenin 3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside; Hellebrigenin triglucoside; NSC 329836; (3β,5β)-3-[(O-6-Deoxy-α-L-mannopyranosyl-(1→2)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranosyl)oxy]-5,14-dihydroxy-19-oxocard-20(22)-enolide
EINECS	Contact for details

Quality Control

Our Hellebrin is produced and handled under strict quality management protocols to ensure batch-to-batch consistency and reliability for research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity determination, spectroscopic identification (IR, NMR), and loss on drying analysis. A detailed Certificate of Analysis (COA) providing full specifications and test results is supplied with every shipment to support your compliance and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, well-ventilated area. Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.