Description

2-Thiocytidine is a chemically modified nucleoside analog where the oxygen atom at the 2-position of the cytidine ribose ring is replaced by a sulfur atom. This structural modification is critical for its role as a key intermediate and building block in the synthesis of oligonucleotides and modified RNA molecules. It is primarily utilized by research institutions and pharmaceutical companies engaged in nucleic acid chemistry, drug discovery, and the development of therapeutic agents. The compound's unique properties make it valuable for probing RNA structure and function and for creating novel bioactive compounds.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite building block for the solid-phase synthesis of thio-modified RNA strands for research and therapeutic applications.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies to investigate RNA metabolism, processing, and protein-RNA interactions.
• Antiviral & Anticancer Drug Development: Serves as a core scaffold for developing nucleoside analog prodrugs targeting viral polymerases or cellular replication machinery.
• Structural Biology: Incorporated into RNA to study folding, stability, and three-dimensional structure using techniques like X-ray crystallography and NMR spectroscopy.
• Diagnostic Probe Development: Used in the design of labeled probes and primers for detecting specific RNA sequences in diagnostic assays.
• RNA Interference (RNAi) Studies: Modification of siRNA or miRNA strands to enhance nuclease resistance and alter pharmacokinetic properties.

Basic Information

Product Name	2-Thiocytidine
CAS No.	13239-97-9
Molecular Formula	C9H13N3O4S
Molecular Weight	259.28 g/mol
Synonyms	2-Thio-cytidine; 2-Thiocytidine; 4-Amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-thiolanyl]-2(1H)-pyrimidinone; 1-(2-Thio-β-D-ribofuranosyl)cytosine; 2'-Thiocytidine; Cytidine, 2-thio-; 2-S-Cytidine
EINECS	Contact for details

Quality Control

Our 2-Thiocytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide Certificates of Analysis (COA) with each shipment, detailing all specifications and test results. Our quality commitment ensures material consistency for critical research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep container tightly sealed in a desiccated environment after opening.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.