Description

Methyl 12Alpha-Hydroxy-3-Oxo-5Beta-Cholan-24-Oate CAS NO 10538-58-6 is a high-purity, synthetic bile acid derivative, specifically a methyl ester of a hydroxylated oxo-cholanic acid. This compound serves as a critical advanced pharmaceutical intermediate and a versatile research-grade biochemical tool for probing metabolic pathways. It is primarily required by pharmaceutical R&D laboratories and fine chemical manufacturers engaged in the synthesis of steroid-based therapeutics and diagnostic agents.

Application

• Pharmaceutical Intermediate: Key building block in the synthetic pathways for novel steroidal drugs and bile acid analogs.
• Biochemical Research: Used as a reference standard or substrate in studies of liver function, cholesterol metabolism, and nuclear receptor (e.g., FXR) signaling.
• Metabolite Synthesis: Precursor for the preparation of specific bile acid metabolites for analytical and diagnostic purposes.
• Academic & Contract Research: Essential for universities and CROs conducting research in gastroenterology, endocrinology, and metabolic diseases.
• Process Development: Serves as a model compound for developing and optimizing chemical synthesis and purification processes in fine chemistry.

Basic Information

Product Name	Methyl 12Alpha-Hydroxy-3-Oxo-5Beta-Cholan-24-Oate
CAS No.	10538-58-6
Molecular Formula	C₂₅H₄₀O₄
Molecular Weight	404.59 g/mol
Synonyms	Methyl 12α-hydroxy-3-oxo-5β-cholan-24-oate; 12α-Hydroxy-3-oxo-5β-cholan-24-oic acid methyl ester; 3-Oxo-12α-hydroxy-5β-cholan-24-oic acid methyl ester; 5β-Cholan-24-oic acid, 12α-hydroxy-3-oxo-, methyl ester; Methyl 3-oxo-12α-hydroxy-5β-cholanate; 12α-Hydroxy-3-oxolithocholic acid methyl ester; Methyl 12α-hydroxy-3-oxolithocholate
EINECS	Contact for details

Quality Control

Our Methyl 12Alpha-Hydroxy-3-Oxo-5Beta-Cholan-24-Oate is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. We provide full traceability and Certificates of Analysis (COA) are available for every shipment, detailing all critical quality attributes. Our standard is tailored to meet the rigorous demands of pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive; prolonged exposure to light should be avoided to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Specific Optical Rotation	Contact for details
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.