Description

25-Hydroxycholest-5-En-3Beta-Yl Acetate is a high-purity sterol derivative, serving as a critical intermediate in advanced pharmaceutical and biochemical research. This compound is valued for its role in the synthesis and study of vitamin D analogs, oxysterols, and other bioactive steroid molecules. It is essential for researchers and manufacturers in the pharmaceutical, life sciences, and fine chemical sectors who require reliable, high-quality building blocks for complex synthetic pathways.

Application

• Pharmaceutical Intermediate: Key starting material or intermediate in the synthesis of vitamin D3 metabolites (calcitriol, calcifediol) and other therapeutic steroid compounds.
• Biochemical Research: Used as a standard or reagent in studies of cholesterol metabolism, oxysterol signaling pathways, and liver X receptor (LXR) biology.
• Reference Standard: Serves as a certified reference material (CRM) for analytical method development and quality control in pharmaceutical laboratories.
• Fine Chemical Synthesis: A versatile chiral building block for the preparation of more complex, functionalized steroid derivatives for various applications.

Basic Information

Product Name	25-Hydroxycholest-5-En-3Beta-Yl Acetate
CAS No.	10525-22-1
Molecular Formula	C₂₉H₄₈O₃
Molecular Weight	444.70 g/mol
Synonyms	25-Hydroxycholesterol 3-acetate; 25-Hydroxycholesteryl acetate; 3β-Acetoxy-25-hydroxycholest-5-ene; Cholest-5-ene-3β,25-diol 3-acetate; 25-HC 3-acetate; (3β)-3-(Acetyloxy)-25-hydroxycholest-5-ene; 25-Hydroxy-3β-acetoxycholest-5-ene
EINECS	Contact for details

Quality Control

Our 25-Hydroxycholest-5-En-3Beta-Yl Acetate is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the stringent requirements for research and synthesis. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive and easily oxidized; for long-term storage, it is recommended to keep the container under an inert atmosphere such as nitrogen or argon.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.