Description

Beta,γ-Methyleneguanosine 5'-Triphosphate Sodium Salt is a chemically modified nucleotide analog, specifically a guanosine triphosphate (GTP) derivative where a methylene bridge links the beta and gamma phosphate groups. This unique structure makes it a valuable non-hydrolyzable GTP analog, crucial for studying GTP-binding proteins (G-proteins) and related enzymatic processes without the risk of hydrolysis. It is primarily utilized by researchers and developers in biochemical research, enzymology, and pharmaceutical development for investigating signal transduction pathways and nucleotide-dependent enzyme mechanisms.

Application

• Biochemical Research: As a stable GTP analog for studying the kinetics and mechanisms of GTPases, including small G-proteins like Ras, Rho, and Rab families.
• Enzymology Studies: Used to probe the active sites of enzymes that utilize GTP as a substrate or cofactor, helping to elucidate reaction mechanisms and inhibition.
• Signal Transduction Research: Essential tool for investigating G-protein coupled receptor (GPCR) pathways and other nucleotide-regulated cellular signaling events.
• Pharmaceutical Screening: Serves as a key reagent in high-throughput screening assays to identify potential therapeutic compounds that target GTP-binding proteins.
• Structural Biology: Used in X-ray crystallography and NMR studies to obtain structural insights into protein-nucleotide complexes.
• Diagnostic Development: Potential use in developing diagnostic kits and assays that monitor GTPase activity in clinical samples.

Basic Information

Product Name	Beta,γ-Methyleneguanosine 5'-Triphosphate Sodium Salt
CAS No.	10470-57-2
Molecular Formula	C11H15N5O13P3•xNa
Molecular Weight	Approx. 605.1 (Free acid), Contact for exact sodium salt form.
Synonyms	β,γ-Methylene-GTP Sodium Salt; Guanosine 5'-[β,γ-methylene]triphosphate Sodium Salt; β,γ-CH2-GTP Na Salt; GMPPCP Sodium Salt; β-γ-Methylene Guanosine Triphosphate Sodium Salt; Non-hydrolyzable GTP analog sodium salt; 5'-Guanylyl methylenediphosphonate Sodium Salt
EINECS	Contact for details.

Quality Control

Our Beta,γ-Methyleneguanosine 5'-Triphosphate Sodium Salt is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR for structural confirmation, and mass spectrometry for identity verification. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant and under inert atmosphere to maintain stability. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Biological Activity	Inhibits GTP hydrolysis in standard assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.