Description

3',5'-Di-o-Acetyl-2'-Deoxy-2'-Fluorouridine is a key fluorinated nucleoside derivative, serving as a critical intermediate in the synthesis of modified oligonucleotides and therapeutic agents. Its primary value lies in its role as a building block for antisense oligonucleotides and siRNA, where the 2'-fluoro modification enhances nuclease resistance and binding affinity. This high-purity compound is essential for pharmaceutical R&D, biotechnology companies, and academic institutions focused on developing novel nucleic acid-based therapeutics and diagnostic probes.

Application

• Pharmaceutical Intermediate: A vital precursor in the synthesis of fluorinated nucleoside analogs for antiviral and anticancer drug development.
• Antisense Oligonucleotide Synthesis: Used as a modified phosphoramidite building block to introduce 2'-fluoro-deoxyribose sugars, enhancing the stability and efficacy of antisense drugs.
• siRNA and RNAi Research: Employed in the manufacture of small interfering RNA (siRNA) strands to improve resistance to degradation and increase potency in gene silencing applications.
• Diagnostic Probe Development: Integral for creating fluorescently labeled or otherwise tagged nucleotide probes used in molecular diagnostics and FISH (Fluorescence In Situ Hybridization).
• Biochemical Research: Serves as a substrate or inhibitor in enzymatic studies to understand polymerase fidelity and nucleic acid metabolism.
• API (Active Pharmaceutical Ingredient) Manufacturing: A controlled intermediate in the cGMP production chain for approved nucleic acid therapeutics.

Basic Information

Product Name	3',5'-Di-o-Acetyl-2'-Deoxy-2'-Fluorouridine
CAS No.	10212-13-2
Molecular Formula	C13H15FN2O7
Molecular Weight	330.27 g/mol
Synonyms	2'-Deoxy-2'-fluorouridine 3',5'-diacetate; 1-[(2R,3R,4R,5R)-3,4-Diacetoxy-5-(fluoromethyl)oxolan-2-yl]pyrimidine-2,4-dione; 2'-Fluoro-2'-deoxyuridine 3',5'-di-O-acetate; 3',5'-Diacetyl-2'-deoxy-2'-fluorouridine; 5'-O-Acetyl-2'-deoxy-2'-fluorouridine 3'-acetate; 2'-F-2'-dUrd diacetate; Fluorouridine diacetate derivative; 2'-Fluoro-5'-O-acetyluridine 3'-acetate
EINECS	Contact for details

Quality Control

Our 3',5'-Di-o-Acetyl-2'-Deoxy-2'-Fluorouridine is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for research and pharmaceutical applications. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles. Quality is verified through techniques including HPLC, NMR, and mass spectrometry. Materials can be supplied to meet specific customer requirements, including standards for use in cGMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.