Description

2'-Deoxyadenosine 5'-Triphosphate (dATP) is a critical deoxyribonucleotide essential for DNA synthesis and replication. As a high-purity biochemical building block, it provides reliable performance in sensitive molecular biology applications where precision is paramount. This product is indispensable for researchers and manufacturers in the biotechnology, pharmaceutical, and molecular diagnostics industries.

Application

• Polymerase Chain Reaction (PCR): Acts as one of the four essential nucleotide substrates for DNA amplification.
• DNA Sequencing: Used in Sanger sequencing and next-generation sequencing (NGS) platforms for accurate base incorporation.
• cDNA Synthesis: Serves as a key component in reverse transcription reactions for first-strand cDNA generation.
• Site-Directed Mutagenesis: Provides the nucleotide triphosphates required for enzymatic DNA synthesis in mutagenesis kits.
• Enzymatic DNA Labeling: Utilized in protocols for incorporating labeled nucleotides for detection and imaging.
• Biochemical Research: Fundamental for in vitro studies of DNA polymerases, kinases, and other nucleic acid-metabolizing enzymes.

Basic Information

Product Name	2'-Deoxyadenosine 5'-Triphosphate
CAS No.	1927-31-7
Molecular Formula	C10H16N5O12P3
Molecular Weight	491.18 g/mol
Synonyms	dATP; Deoxyadenosine triphosphate; 2'-Deoxy-ATP; Adenosine 5'-(tetrahydrogen triphosphate), 2'-deoxy-; dATP trisodium salt (common salt form); 2'-Deoxyadenosine-5'-triphosphate; Deoxyadenosine 5'-(tetrahydrogen triphosphate)
EINECS	217-682-2

Quality Control

Our 2'-Deoxyadenosine 5'-Triphosphate is manufactured under stringent quality systems to ensure batch-to-batch consistency suitable for research and diagnostic applications. Each lot is tested to meet high-purity standards, with comprehensive analysis for identity, potency, and the absence of critical contaminants like nucleases. Certificates of Analysis (COA) detailing purity, HPLC profile, and other relevant specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Assay (HPLC)	≥ 95%
Purity (HPLC)	≥ 98% (Area %)
pH (in solution)	6.0 - 7.5
Water Content (KF)	≤ 5.0 %
Endotoxin	< 10 EU/mg (if specified for molecular biology grade)
DNase/RNase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.