Description

o-Trimethylsilylcholesterol is a silylated derivative of cholesterol, specifically functionalized at the 3β-hydroxyl position with a trimethylsilyl (TMS) protecting group. This modification is critical for enhancing the compound's volatility and thermal stability, making it an essential intermediate in analytical chemistry and organic synthesis. It is primarily utilized by research institutions, pharmaceutical development labs, and specialty chemical manufacturers involved in sterol analysis, derivatization for GC/MS, and the synthesis of complex steroid-based molecules.

Application

• Gas Chromatography-Mass Spectrometry (GC/MS) Analysis: Used as a derivatization agent for cholesterol and related sterols to improve their chromatographic separation, peak shape, and detection sensitivity.
• Pharmaceutical Research & Development: Serves as a key protected intermediate in the multi-step synthesis of novel steroid drugs and bioactive molecules.
• Biomarker Analysis: Facilitates the accurate quantification of cholesterol and its metabolites in clinical and biological samples for diagnostic and research purposes.
• Reference Standard: Employed as a high-purity analytical standard for method development and validation in quality control laboratories.
• Material Science Research: Investigated for the synthesis of functionalized sterol-based materials, such as liquid crystals or surface-modifying agents.
• Metabolic Pathway Studies: Aids in tracing and analyzing sterol transformation pathways in biochemical research.

Basic Information

Item	Details
Product Name	o-Trimethylsilylcholesterol
CAS No.	1856-05-9
Molecular Formula	C30H54OSi
Molecular Weight	458.85 g/mol
Synonyms	Cholesterol TMS Ether; 3β-[(Trimethylsilyl)oxy]cholest-5-ene; 5-Cholesten-3β-ol, trimethylsilyl ether; Trimethylsilyl cholesterol ether; TMS-Cholesterol; Cholesterol trimethylsilyl ether; Cholest-5-en-3β-ol, 3-trimethylsilyl ether; NSC 334288
EINECS	Contact for details

Quality Control

Our o-Trimethylsilylcholesterol is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity. Each lot is subjected to comprehensive analytical testing, including chromatographic purity assessment and structural confirmation. A Certificate of Analysis (COA) detailing specific results for identity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is moisture-sensitive; keep the container tightly sealed in a dry environment.

Specification

Item	Specification
Appearance	White to off-white crystalline powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.