Description

Pseudouridine 5’-Triphosphate (ψTP) is a critical modified nucleotide triphosphate essential for advanced RNA research and therapeutic development. Its primary value lies in enhancing the stability and reducing the immunogenicity of synthetic mRNA, a cornerstone technology for vaccines and gene therapies. This high-purity biochemical is indispensable for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require reliable, high-quality raw materials for cutting-edge applications.

Application

• mRNA Vaccine & Therapeutic Manufacturing: Used as a key component to incorporate pseudouridine into synthetic mRNA strands, improving translational efficiency and stability while minimizing unwanted immune responses.
• RNA Biochemistry & Enzymology Research: Serves as a substrate for RNA polymerases (e.g., T7 RNA polymerase) in in vitro transcription to study RNA structure, function, and pseudouridylation mechanisms.
• Synthetic Biology & tRNA Engineering: Employed in the enzymatic synthesis of modified tRNAs to investigate and enhance protein translation systems.
• Diagnostic Probe Development: Utilized in the synthesis of labeled RNA probes and primers that require increased nuclease resistance for sensitive detection assays.
• Antisense Oligonucleotide & siRNA Research: Incorporated into oligonucleotides to modulate RNA interference (RNAi) activity and improve pharmacokinetic properties.
• Cell-Free Protein Synthesis Systems: A vital reagent in optimized cell-free platforms for high-yield production of proteins containing non-canonical amino acids.

Basic Information

Item	Detail
Product Name	Pseudouridine 5’-Triphosphate
CAS No.	1175-34-4
Molecular Formula	C9H15N2O15P3
Molecular Weight	508.14 g/mol
Synonyms	ψTP; Pseudouridine-5'-triphosphate; 5-Triphosphoric acid pseudouridine; 5-(Trihydroxyphosphinoxy)phosphonic acid pseudouridine; 1-β-D-Ribofuranosyl-2,4(1H,3H)-pyrimidinedione 5'-(tetrahydrogen triphosphate); Pseudouridylic acid triphosphate; 5-Triphosphoric acid 5-ribosyluracil
EINECS	Contact for details

Quality Control

Our Pseudouridine 5’-Triphosphate is manufactured under strict quality systems to ensure it meets the exacting requirements of life science research and GMP-grade applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS). A detailed Certificate of Analysis (COA) providing full specifications and test results is provided with every shipment to guarantee traceability, consistency, and compliance with your internal quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 5.0%
Solubility	Clear, colorless solution in water or buffer
Biological Activity	Suitable for in vitro transcription

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.