Description

2'-Deoxyuridine-5'-Triphosphoric Acid (dUTP) is a critical nucleotide triphosphate essential for molecular biology research and diagnostic applications. Its primary value lies in its role as a substrate for DNA polymerases, enabling controlled DNA synthesis and labeling. This compound is indispensable for researchers and manufacturers in the fields of molecular diagnostics, PCR, DNA sequencing, and biopharmaceutical development.

Application

• DNA Polymerase Substrate: Essential building block for in vitro DNA synthesis, replication, and amplification techniques.
• Polymerase Chain Reaction (PCR): Used in specialized PCR applications, including mutagenesis and the incorporation of labeled nucleotides.
• DNA Sequencing: Serves as a natural substrate in Sanger sequencing and next-generation sequencing (NGS) workflows.
• Molecular Diagnostics: A key component in assay kits for detecting viral DNA, genetic mutations, and other biomarkers.
• Biochemical Research: Utilized in enzyme kinetics studies, DNA repair mechanism investigations, and apoptosis research.
• Labeled Probe Synthesis: Acts as a precursor for the enzymatic incorporation of digoxigenin, biotin, or fluorescent tags into DNA probes.
• Cell Biology Studies: Employed in techniques like TUNEL assay for detecting DNA fragmentation and programmed cell death.

Basic Information

Item	Detail
Product Name	2'-Deoxyuridine-5'-Triphosphoric Acid (dUTP)
CAS No.	1173-82-6
Molecular Formula	C9H15N2O14P3
Molecular Weight	468.14 g/mol
Synonyms	dUTP; 2'-Deoxyuridine 5'-triphosphate; Deoxyuridine triphosphate; Deoxyuridine-5'-triphosphate; dUTP, trisodium salt; Uridine, 2'-deoxy-, 5'-(tetrahydrogen triphosphate); 5'-dUTP; 2'-Deoxyuridine-5'-triphosphoric acid
EINECS	214-593-9

Quality Control

Our 2'-Deoxyuridine-5'-Triphosphoric Acid is manufactured under stringent quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for concentration verification, and enzymatic activity assays, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) detailing purity, identity, and performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate and keep frozen. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Anhydrous basis)
Assay (Enzymatic)	≥ 90%
pH (1% Solution)	6.0 - 7.5
Water Content (KF)	≤ 5.0 %
Endotoxin	< 10 EU/mg (for molecular biology grade)
DNase/RNase Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.