Description

2,4(1H,3H)-Pyrimidinedione, 1-[(5E)-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-5,6-dideoxy-6-(diethoxyphosphinyl)-2-O-methyl-β-D-ribo-hex-5-enofuranosyl]- is a sophisticated nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. This high-purity intermediate is essential for ensuring the fidelity and yield of custom DNA and RNA sequences. It is primarily utilized by research institutions, diagnostic developers, and pharmaceutical companies engaged in therapeutic oligonucleotide development, genetic research, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, stepwise assembly of DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for targeted drug therapies.
• Research Probe and Primer Synthesis: Creation of labeled or modified probes for PCR, sequencing, and hybridization assays.
• Diagnostic Kit Component: Synthesis of capture probes and primers used in clinical diagnostic tests and genomic analysis.
• Antisense Technology Development: Fundamental research and development of gene silencing and modulation technologies.
• Synthesis of Modified Nucleotides: For incorporating specific functionalities or stability enhancements into nucleic acid constructs.

Basic Information

Item	Detail
Product Name	2,4(1H,3H)-Pyrimidinedione, 1-[(5E)-3-O-[[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-5,6-dideoxy-6-(diethoxyphosphinyl)-2-O-methyl-β-D-ribo-hex-5-enofuranosyl]-
CAS No.	2361324-80-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methyluridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-Methyluridine Phosphoramidite; 2'-OMe-U Phosphoramidite; 2'-O-Methyl-5'-O-DMT-uridine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); Uridine, 2'-O-methyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 2'-O-Methyl-5'-O-DMT-uridine CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are produced under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is characterized by advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive and light-sensitive nature. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.