Description

3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt is a specialized, high-purity functional dye derivative belonging to the cyanine dye family, designed for advanced bioconjugation and labeling applications. This compound matters for its unique combination of a reactive azide group and sulfonate moieties, enabling efficient, bio-orthogonal click chemistry for labeling biomolecules while maintaining excellent water solubility. It is critically needed by researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and advanced materials science who require precise, stable, and fluorescent probes for imaging, detection, and analysis.

Application

• Bioconjugation and Probe Labeling: Utilized as a fluorescent label for proteins, antibodies, nucleic acids, and other biomolecules via copper-free click chemistry.
• Bioimaging and Diagnostics: Serves as a key component in the development of fluorescent probes for in vitro and in vivo cellular imaging, flow cytometry, and immunohistochemistry.
• Pharmaceutical Research: Used in drug discovery for target identification, ligand-receptor binding studies, and high-throughput screening assays.
• Diagnostic Kit Manufacturing: Incorporated into lateral flow assays, ELISA kits, and other point-of-care diagnostic devices for sensitive detection.
• Materials Science: Applied in the surface functionalization of nanoparticles and polymers to create smart, fluorescent materials for sensing applications.
• Life Science Research: Essential tool for molecular biology and biochemistry research involving fluorescence resonance energy transfer (FRET) and super-resolution microscopy.

Basic Information

Product Name	3H-IndoliuM, 2-[5-[1-[6-[(3-azidopropyl)aMino]-6-oxohexyl]-1,3-dihydro-3,3-diMethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1,3,3-triMethyl-5-sulfo-, inner salt
CAS No.	1481447-40-8
Molecular Formula	C41H53N6O7S2+ • (Inner Salt)
Molecular Weight	Approx. 789.02 g/mol (Contact for details)
Synonyms	Sulfo-Cyanine5 azide; Cy5 azide, sulfonated; Azide-functionalized Cy5 dye; Water-soluble Cy5-N3; 3-Azidopropylamino-Cy5; Pentamethine cyanine dye azide; Near-infrared fluorescent azide; CAS 1481447-40-8
EINECS	Contact for details

Quality Control

Our production of this high-value functional dye adheres to strict quality management protocols to ensure batch-to-batch consistency and performance reliability. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for functional validation of absorbance and fluorescence properties. A detailed Certificate of Analysis (COA) documenting all critical quality attributes is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For prolonged storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0 %
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Functional Group Integrity	Azide content confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.