Description

Propidium Iodide is a vital fluorescent dye and nucleic acid stain widely used in life science research. Its core value lies in its ability to selectively bind to DNA and RNA, making it an indispensable tool for cell viability analysis and flow cytometry. This compound is essential for researchers and quality control professionals in biotechnology, pharmaceutical development, and clinical diagnostics. Propidium Iodide CAS NO 25535-16-4 is a cornerstone reagent for applications requiring the discrimination between live and dead cells.

Application

• Cell Viability and Cytotoxicity Assays: Primary stain for distinguishing live cells from dead or membrane-compromised cells in microscopy and plate reader formats.
• Flow Cytometry (FACS): Critical for DNA content/cell cycle analysis and for excluding dead cells from analysis to ensure accurate results.
• Fluorescence Microscopy: Used to visualize nuclei in fixed cells or in live-cell imaging protocols where membrane integrity is assessed.
• Apoptosis Detection: Employed in combination with Annexin V in assays to identify early and late apoptotic cell populations.
• Nucleic Acid Staining in Gel Electrophoresis: Alternative to ethidium bromide for visualizing DNA bands in agarose gels.
• Bacterial Viability Testing: Applied in microbiology to assess bacterial cell membrane integrity.
• Sorting of Fixed Cells: Enables the sorting of cell populations based on DNA content after fixation procedures.
• Research in Neuroscience and Cancer Biology: Utilized in studies involving neuronal death, tumor biology, and screening of chemotherapeutic agents.

Basic Information

Product Name	Propidium Iodide
CAS No.	25535-16-4
Molecular Formula	C27H34I2N4
Molecular Weight	668.39 g/mol
Synonyms	3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide; PI; Propidium Iodide; Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-, diiodide; Propidium diiodide; Cell Viability Stain; CAS 25535-16-4
EINECS	247-081-2

Quality Control

Our Propidium Iodide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, absorbance/fluorescence spectral verification, and functional testing in cell-based assays. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, identity, and performance specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	Dark red to red-brown crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0% (Area %)
Absorbance (Max)	493 nm, 536 nm (in aqueous solution)
Emission (Max)	~617 nm (with DNA binding, ex. 536 nm)
Loss on Drying	≤ 2.0 %
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.