Description

Trypan Blue is a vital azo dye widely recognized for its selective staining properties in biological assays. Its core value lies in its ability to differentiate between viable and non-viable cells, a critical parameter in cell culture, viability testing, and biomedical research. This product is essential for professionals in the biotechnology, pharmaceutical, and academic research sectors who require reliable, high-quality reagents for diagnostic and experimental protocols. We supply Trypan Blue CAS NO 72-57-1 to meet the stringent demands of global industrial and research applications.

Application

• Cell Viability and Cytotoxicity Assays: The primary application for distinguishing live from dead cells in suspension cultures.
• Microscopy and Histology: Used as a contrast stain for background tissue in frozen sections and for visualizing yeast and mold.
• Blood Cell Analysis: Employed in manual hemocytometer counts to assess white blood cell viability.
• In vitro Diagnostics (IVD): A key component in diagnostic kits and reagents for clinical laboratory testing.
• Biopharmaceutical Production: Monitors cell viability during upstream bioprocessing and cell line development.
• Academic & Industrial Research: Fundamental tool in molecular biology, immunology, and cancer research laboratories.

Basic Information

Product Name	Trypan Blue
CAS No.	72-57-1
Molecular Formula	C34H24N6Na4O14S4
Molecular Weight	960.81 g/mol
Synonyms	Trypan Blue; Direct Blue 14; Benzamine Blue 3B; Niagara Blue 3B; Congo Blue; Azidine Blue 3B; C.I. 23850; Diamine Blue 3B; Trypanblau; 3,3'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid] tetrasodium salt
EINECS	200-786-7

Quality Control

Our Trypan Blue is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and industrial use. A Certificate of Analysis (COA) detailing specific results for purity, absorbance, and other critical parameters is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at a controlled room temperature (15-25°C) in a dry, well-ventilated area. Keep away from heat and incompatible materials.

Specification

Item	Specification
Appearance	Dark blue to blue-black powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 85% (Dye content)
Absorbance (1% solution)	Characteristic peak at ~607 nm
Loss on Drying	≤ 10.0 %
Water Solubility	Soluble (1% solution should be clear)
pH (1% solution)	6.0 - 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.