Description

Methylene Blue Trihydrate CAS NO 7220-79-3 is a vital organic salt and thiazine dye, widely recognized for its distinctive blue color and redox properties. This compound is essential for a multitude of industrial, research, and diagnostic applications due to its high purity and consistent performance. It is a critical reagent for laboratories, pharmaceutical manufacturers, and water treatment facilities requiring a reliable biological stain, redox indicator, or therapeutic agent.

Application

• Biological Staining: Used as a vital stain in microbiology and histology for cell and tissue visualization.
• Medical & Pharmaceutical: Employed as an antidote for methemoglobinemia and in the formulation of certain medicinal products.
• Analytical Chemistry: Serves as a redox indicator in titrations and in biochemical assays to measure metabolic activity.
• Aquaculture & Aquarium Industry: Applied as a treatment for fungal and parasitic infections in fish.
• Textile Dyeing: Functions as a dye for silk, wool, and other materials, though largely replaced by synthetic alternatives.
• Research & Development: Utilized in various scientific studies, including photodynamic therapy research and as a model compound in chemistry.

Basic Information

Product Name	Methylene Blue Trihydrate
CAS No.	7220-79-3
Molecular Formula	C16H18ClN3S · 3H2O
Molecular Weight	373.90 g/mol (Trihydrate)
Synonyms	Methylthioninium Chloride Trihydrate; Basic Blue 9 Trihydrate; 3,7-Bis(Dimethylamino)phenothiazin-5-ium Chloride Trihydrate; Swiss Blue; Urolene Blue; CI 52015; Methylenum Caeruleum; Tetramethylthionine Chloride
EINECS	200-515-2 (for the anhydrous form)

Quality Control

Our Methylene Blue Trihydrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for laboratory and industrial use. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). Keep away from incompatible materials. Due to its light-sensitive nature, prolonged exposure to direct light should be avoided to maintain stability.

Specification

Item	Specification
Appearance	Dark green crystalline powder with bronze luster
Identification (IR)	Conforms to standard
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Loss on Drying	13.0 - 16.0 %
Water (Karl Fischer)	13.0 - 16.0 %
Residue on Ignition	≤ 0.1 %
Heavy Metals (as Pb)	≤ 20 ppm
Absorbance Ratio (A664/A610)	≥ 1.15

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.