Description

4',6-Diamidino-2-Phenylindole Dihydrochloride is a high-purity, cell-permeable fluorescent dye widely used as a nuclear and chromosome counterstain. Its core value proposition lies in its strong, specific binding to adenine-thymine (A-T) rich regions of double-stranded DNA, producing a significant fluorescence enhancement ideal for sensitive detection. This product is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and life science sectors requiring reliable nucleic acid visualization.

Application

• Fluorescence Microscopy: Primary application for staining nuclei in fixed cells and tissue sections, providing excellent contrast in multicolor imaging.
• Flow Cytometry: Used for DNA content quantification and cell cycle analysis due to its stoichiometric DNA binding.
• Molecular Biology: Detection of DNA in agarose and polyacrylamide gels following electrophoresis.
• Virology & Microbiology: Staining of viral plaques and bacterial colonies for enumeration and visualization.
• Apoptosis Assays: Employed to identify apoptotic cells with condensed or fragmented nuclei.
• Chromosome Banding: Useful in cytogenetics for producing DAPI-banding patterns on chromosomes.
• High-Content Screening (HCS): A robust nuclear marker in automated imaging systems for drug discovery and toxicology studies.
• Forensic Science: Applied for the detection of human cellular material and DNA in forensic samples.

Basic Information

Product Name	4',6-Diamidino-2-Phenylindole Dihydrochloride
CAS No.	28718-90-3
Molecular Formula	C16H15N5 · 2HCl
Molecular Weight	350.25 g/mol (Free base: 277.32 g/mol)
Synonyms	DAPI Dihydrochloride; 4',6-Diamidino-2-phenylindole dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarboxamidine dihydrochloride; DAPI; Dihydrochloride, 4',6-Diamidino-2-Phenylindole; DAPI Stain; 28718-90-3; DAPI, dihydrochloride
EINECS	Contact for details

Quality Control

Our 4',6-Diamidino-2-Phenylindole Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectral analysis for identity confirmation, and tests for residual solvents and moisture content, to ensure it meets the high standards required for critical research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
UV-Vis Absorption	λmax ~358 nm (in methanol)
Fluorescence Emission	λmax ~461 nm (when bound to DNA)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.