Description

Hematoxylin CAS NO 517-28-2 is a natural phenolic compound extracted from the heartwood of the logwood tree (*Haematoxylum campechianum*). It serves as the foundational staining agent in histology and cytology, forming the basis of the widely used Hematoxylin and Eosin (H&E) stain. This product is essential for laboratories and manufacturers in the life sciences, diagnostic, and educational sectors, where it is used to prepare histological stains for visualizing cell nuclei and other basophilic structures in tissue samples.

Application

• Histological Staining: Primary use as a nuclear stain in the preparation of Hematoxylin and Eosin (H&E) stains for microscopic tissue analysis in pathology, histology, and biomedical research.
• Cytological Staining: Used in Papanicolaou (Pap) stains and other cytological procedures for examining cell samples.
• Special Stains: A key component in more complex staining protocols, such as Masson's trichrome, Verhoeff's stain, and iron hematoxylin, for differentiating connective tissues, elastin, and other specific cellular components.
• Diagnostic Reagent Manufacturing: Supplied as a raw material to in-vitro diagnostic (IVD) and clinical chemistry manufacturers for producing standardized staining kits and solutions.
• Botanical & Educational Use: Employed in plant biology for staining plant tissues and in educational settings for teaching basic histological techniques.
• Research & Development: Utilized in life science R&D for developing new staining methodologies and studying cellular morphology.

Basic Information

Product Name	Hematoxylin
CAS No.	517-28-2
Molecular Formula	C16H14O6
Molecular Weight	302.28 g/mol
Synonyms	Natural Black 1; C.I. 75290; Haematoxylin; Hydroxybrasilin; 7,11b-Dihydrobenz[b]indeno[1,2-d]pyran-3,4,6a,9,10(6H)-pentol; Hematoxiline; Campeachy Wood Extract; Logwood Extract; Hematoxylin (natural)
EINECS	208-237-3

Quality Control

Our Hematoxylin is produced and tested under a rigorous quality management system. Each batch is analyzed to ensure it meets stringent specifications for identity, purity, and performance in staining applications. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and physical characteristics is provided with every shipment to guarantee traceability and consistency for your critical laboratory and manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability and performance.

Specification

Item	Specification
Appearance	Light yellow to brown crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition (Sulfated Ash)	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Staining Performance Test	Passes (vs. reference standard)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.