Description

Phosphoramidous acid, N,N-bis(1-methylethyl)-, (2R)-2-[(1R)-1-[4-(acetylamino)-2-oxo-1(2H)-pyrimidinyl]-2-(benzoyloxy)ethoxy]-3-[bis(4-methoxyphenyl)phenylmethoxy]propyl 2-cyanoethyl ester is a sophisticated, high-purity phosphoramidite monomer, a critical building block in automated oligonucleotide synthesis. Its value lies in enabling the precise and efficient incorporation of modified nucleosides, specifically an N4-acetylcytidine derivative, into synthetic DNA and RNA strands. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, antisense technology, and diagnostic probe synthesis, where sequence-specific modifications are required for stability, targeting, and efficacy.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite reagent for the automated synthesis of DNA/RNA sequences containing modified cytidine bases.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense oligonucleotides (ASOs), siRNA, and aptamers with enhanced nuclease resistance and binding affinity.
• Research & Diagnostic Probe Development: Synthesis of labeled or modified probes for genomics, PCR, FISH, and other molecular biology applications requiring specific hybridization properties.
• Synthesis of Modified Nucleosides: Used in the preparation of nucleoside analogs for biochemical and virological studies.
• Precision Medicine Tools: Creation of custom oligonucleotides for target validation, gene silencing, and personalized therapeutic approaches.

Basic Information

Item	Detail
Product Name	Phosphoramidous acid, N,N-bis(1-methylethyl)-, (2R)-2-[(1R)-1-[4-(acetylamino)-2-oxo-1(2H)-pyrimidinyl]-2-(benzoyloxy)ethoxy]-3-[bis(4-methoxyphenyl)phenylmethoxy]propyl 2-cyanoethyl ester
CAS No.	1120329-56-7
Molecular Formula	C55H62N5O11P
Molecular Weight	1000.09 g/mol
Synonyms	N4-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(tert-butyldimethylsilyl)]-cytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Ac4C Phosphoramidite; 5'-DMT-2'-TBDMS-N4-acetylcytidine-3'-CE phosphoramidite; N4-Acetyl-2'-O-TBDMS-cytidine CE phosphoramidite; Modified Cytidine Phosphoramidite; RNA Phosphoramidite (Ac4C); Oligonucleotide Synthesis Reagent
EINECS	Contact for details

Quality Control

This high-value synthesis reagent is produced under strict quality-controlled conditions to ensure batch-to-batch consistency and reliability for sensitive automated synthesis platforms. Each lot is characterized by HPLC, NMR, and MS analysis to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling efficiency tests is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dedicated freezer. Allow the sealed vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation. This material is both moisture-sensitive and easily oxidized (store under inert atmosphere).

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Coupling Efficiency Test	≥ 98.5% step-wise yield

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.