Description

Biotin (5-Fluorescein) Conjugate is a high-purity, fluorescently labeled derivative of biotin, designed for advanced detection and analysis. This compound is essential for creating highly sensitive and specific detection systems in biochemical research and diagnostic development. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and in vitro diagnostics (IVD) industries for applications such as immunoassays, fluorescence microscopy, and flow cytometry.

Application

• Immunoassay Development: Acts as a fluorescent tracer in ELISA, Western blotting, and lateral flow assays for the detection of biotinylated antibodies or other molecules.
• Fluorescence Microscopy & Imaging: Used to label and visualize cellular components, proteins, or nucleic acids that have been tagged with streptavidin or avidin.
• Flow Cytometry: Serves as a critical fluorescent probe for the detection and sorting of cells labeled with biotin-conjugated ligands.
• Diagnostic Kit Manufacturing: A key component in the production of commercial diagnostic kits requiring high-sensitivity fluorescence detection.
• Biomolecular Interaction Studies: Employed in techniques like fluorescence polarization (FP) or fluorescence resonance energy transfer (FRET) to study binding kinetics and molecular interactions.
• Nucleic Acid Detection: Utilized in fluorescence in situ hybridization (FISH) and other genomic analysis methods when paired with biotinylated probes.

Basic Information

Product Name	Biotin (5-Fluorescein) Conjugate
CAS No.	957494-27-8
Molecular Formula	C37H38N4O8S
Molecular Weight	698.79 g/mol
Synonyms	5-Fluorescein Biotin; Biotin-5-Fluorescein; Biotin-FITC; Fluorescein Biotin Conjugate; Biotinamidohexanoyl-6-aminohexanoic-fluorescein; N-(6-(Biotinamido)hexyl)-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxamide; 5-FAM Biotin; Biotin, conjugate with fluorescein
EINECS	Contact for details

Quality Control

Every batch of our Biotin (5-Fluorescein) Conjugate is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure high purity and consistent performance through methods including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) providing batch-specific data on identity, purity, and fluorescence properties is supplied with each order.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~68,000 cm-1M-1 at 494 nm
Fluorescence Emission	~518 nm
Solubility	DMSO, DMF
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.