Description

2-(2-Aminoethyl) Rhodamine B Amide is a chemically reactive derivative of the classic Rhodamine B fluorophore, engineered for advanced bioconjugation and labeling applications. This compound is critical for researchers and manufacturers who require a highly fluorescent, amine-reactive probe for tagging biomolecules such as proteins, peptides, and oligonucleotides. It is primarily utilized in the life sciences, diagnostics, and materials science sectors for applications ranging from fluorescence microscopy and flow cytometry to the development of novel sensor materials.

Application

• Bioconjugation and Fluorescent Labeling: Primary application for covalent attachment to proteins, antibodies, and other biomolecules via its primary amine group.
• Cell Biology and Imaging: Used as a vital stain in fluorescence microscopy (e.g., confocal, super-resolution) for visualizing cellular structures and tracking intracellular processes.
• Flow Cytometry: Serves as a bright fluorescent tag for antibody conjugates in immunophenotyping and cell sorting applications.
• Diagnostic Assay Development: Key component in the fabrication of fluorescent probes and biosensors for clinical diagnostics and high-throughput screening.
• Materials Science: Incorporation into polymers, nanoparticles, and hydrogels to create smart, fluorescent materials for sensing and imaging.
• Chemical Biology Research: Tool for studying protein-protein interactions, localization, and dynamics in live or fixed cells.

Basic Information

Product Name	2-(2-Aminoethyl) Rhodamine B Amide
CAS No.	950846-89-6
Molecular Formula	C32H37N4O3+ (typically as a salt, e.g., trifluoroacetate)
Molecular Weight	525.67 g/mol (for free base cation)
Synonyms	Rhodamine B 2-(2-Aminoethyl)amide; N-(2-Aminoethyl)rhodamine B amide; 5-Carboxyrhodamine 110 2-aminoethylamide; Rhodamine B ethylenediamine amide; Reactive Rhodamine B derivative; Aminoethyl Rhodamine B; X-Rhodamine amine; [9-(2-Carboxy-2-aminoethyl-carbonyl)phenyl]-6-diethylamino-3H-xanthen-3-ylidene]-diethylammonium
EINECS	Contact for details

Quality Control

Our 2-(2-Aminoethyl) Rhodamine B Amide is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance data. We support compliance with relevant research and development standards, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Absorption (λmax)	~570 nm
Emission (λmax)	~595 nm
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 2.0 %
Functional Performance	Conjugation efficiency verified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.