Description

5-Tamra Alkyner is a specialized fluorescent labeling reagent, a derivative of the widely used carboxytetramethylrhodamine (TAMRA) dye, functionalized with an alkyne group for bioorthogonal click chemistry. This compound is critical for researchers and developers requiring precise, covalent tagging of biomolecules such as proteins, nucleic acids, and glycans in complex biological systems. It is primarily utilized in advanced life science research, pharmaceutical development, and diagnostic assay design, enabling high-sensitivity detection and imaging.

Application

• Bioorthogonal Labeling: Covalent attachment to azide-modified biomolecules via copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) click reactions.
• Fluorescent Probes & Sensors: Synthesis of targeted fluorescent probes for cellular imaging, flow cytometry, and fluorescence microscopy.
• Proteomics & Biomarker Discovery: Labeling of proteins and peptides for activity-based protein profiling (ABPP) and pull-down assays.
• Nucleic Acid Labeling: Preparation of fluorescently tagged oligonucleotides for FISH, PCR, and next-generation sequencing applications.
• Glycan Imaging: Metabolic labeling and visualization of cellular glycans for glycobiology studies.
• Diagnostic Reagent Development: Key component in the manufacture of fluorescent lateral flow assays and in vitro diagnostic (IVD) kits.
• Drug Delivery & Conjugate Chemistry: Facilitating the tracking and study of drug delivery systems and antibody-drug conjugates (ADCs).

Basic Information

Product Name	5-Tamra Alkyner
CAS No.	945928-17-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-Carboxytetramethylrhodamine Alkyne; 5-TAMRA Alkyne; 5-TAMRA-PEG4-Alkyne; TAMRA C5 Alkyne; 6-Carboxy-tetramethylrhodamine-propargylamide; TAMRA Alkyne, 5-isomer; Rhodamine-based Alkyne Tag; 5/6-TAMRA Alkyne (mixture may be specified)
EINECS	Contact for details

Quality Control

Our 5-Tamra Alkyner is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. Keep container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion pattern
Purity (HPLC)	≥ 95% (Area % at 550 nm)
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.