Description

Cyanine 3 Monofunctional Hexanoic Acid Dye, Succinimidyl Ester, Potassium Salt 85% is a reactive near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This compound is essential for researchers and manufacturers requiring high-sensitivity detection and imaging in complex biological systems. It is primarily used by professionals in the biotechnology, pharmaceutical, and diagnostic industries for applications such as antibody labeling, nucleic acid detection, and in vivo imaging.

Application

• Antibody and Protein Labeling: Conjugation to primary amines for fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and Western blotting.
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH), real-time PCR, and microarray analysis.
• In Vivo Imaging: Development of targeted imaging agents for preclinical research in oncology and other disease models.
• Diagnostic Assay Development: Creation of fluorescent conjugates for lateral flow assays, ELISA, and other clinical diagnostic platforms.
• Biomaterial and Nanoparticle Tagging: Functionalization of surfaces, polymers, and nanoparticles for tracking and biodistribution studies.
• Proteomics and Biomarker Discovery: Fluorescent tagging of peptides and proteins for gel-based and liquid chromatography separation and analysis.

Basic Information

Product Name	Cyanine 3 Monofunctional Hexanoic Acid Dye, Succinimidyl Ester, Potassium Salt 85%
CAS No.	945529-56-6
Molecular Formula	C37H45KN2O6
Molecular Weight	652.88 g/mol
Synonyms	Cy3 NHS ester, potassium salt; Cyanine3 succinimidyl ester; Sulfo-Cy3 NHS ester (potassium salt variant); 1-(6-((E)-2-((E)-3,3-Dimethyl-5-sulfo-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)indolin-2-ylidene)ethylidene)-2-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)ethylidene)-3,3-dimethyl-5-sulfoindolin-1-ium-1-yl)hexane-6-carboxylate, potassium salt; Cy3™ Mono NHS Ester; Cy3 carboxylic acid, succinimidyl ester, potassium salt; Near-IR fluorescent dye NHS ester
EINECS	Contact for details

Quality Control

Our Cyanine 3 Monofunctional Dye is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity profile, spectrophotometry for labeling efficiency verification, and functional testing in standard conjugation assays. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This material is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize condensation and hydrolysis of the active ester.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 85.0% (Cy3 active ester)
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Free Amine	≤ 2.0%
Water Content (KF)	≤ 5.0%
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.