Description

Rhodamine B Isothiocyanate (Mixed Isomers) is a reactive fluorescent dye derivative used for labeling proteins, antibodies, and other biomolecules. This compound is critical for creating highly sensitive and specific fluorescent probes for detection and imaging applications. It is primarily needed by researchers and manufacturers in the fields of biotechnology, diagnostics, and life sciences for applications such as fluorescence microscopy, flow cytometry, and immunoassay development.

Application

• Fluorescent labeling of proteins, peptides, and antibodies for detection and quantification.
• Preparation of fluorescent conjugates for use in fluorescence microscopy and cell imaging.
• Development of probes for flow cytometry to analyze and sort cell populations.
• Manufacturing of diagnostic reagents for immunoassays (e.g., ELISA, Western Blot).
• Labeling of oligonucleotides and nucleic acids for in situ hybridization (FISH) techniques.
• Use as a tracer in environmental and industrial testing where sensitive detection is required.

Basic Information

Product Name	Rhodamine B Isothiocyanate (Mixed Isomers)
CAS No.	944130-99-8
Molecular Formula	C29H29N3O3S
Molecular Weight	507.62 g/mol
Synonyms	Rhodamine B Isothiocyanate; Rhodamine B ITC; RBITC; 5(6)-Isothiocyanato-rhodamine B; Rhodamine B isothiocyanate isomer mixture; Rhodamine B isothiocyanate, mixed isomers; Lissamine Rhodamine B sulfonyl chloride; Sulforhodamine B isothiocyanate
EINECS	Contact for details

Quality Control

Our Rhodamine B Isothiocyanate (Mixed Isomers) is produced under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and performance consistency for sensitive labeling applications. Certificates of Analysis (COA) detailing purity, isomer ratio, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Mixed Isomers)
Isomer Ratio	Specified on COA
Extinction Coefficient	Contact for details
Fluorescence	Emits in orange-red region (~580 nm)
Solubility	Soluble in DMF, DMSO, acetonitrile
Moisture Content (KF)	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.