Description

Sulfo-Cy5 NHS Methyl Type is a high-performance, water-soluble cyanine dye derivative, specifically engineered for efficient bioconjugation. This compound is a critical reagent for labeling biomolecules, offering superior stability and fluorescence properties for sensitive detection. It is essential for researchers and developers in the fields of biotechnology, pharmaceutical discovery, and in vitro diagnostics who require reliable, high-quality fluorescent probes for assays, imaging, and diagnostic applications.

Application

• Antibody and Protein Labeling: Covalent conjugation to primary amines (-NH2) for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and Western blotting.
• Nucleic Acid Probes: Labeling of oligonucleotides, primers, and probes for real-time PCR (qPCR), fluorescence in situ hybridization (FISH), and microarray analysis.
• Biomolecular Imaging: Preparation of fluorescent tracers for in vivo and in vitro imaging studies, including near-infrared (NIR) fluorescence applications.
• Diagnostic Assay Development: Key component in the manufacturing of lateral flow assays (LFAs), ELISA kits, and other point-of-care diagnostic tools.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) assays to label targets for binding and inhibition studies.
• Biomaterial and Nanoparticle Functionalization: Conjugation to surfaces of polymers, beads, or nanoparticles to create fluorescently tagged materials for tracking and analysis.

Basic Information

Product Name	Sulfo-Cy5 NHS Methyl Type
CAS No.	937739-58-7
Molecular Formula	C41H47KN4O10S2
Molecular Weight	883.06 g/mol
Synonyms	Sulfo-Cyanine5 NHS ester; Sulfo-Cy5 NHS ester; Cyanine5 sulfonyl NHS ester; Sulfo-Cy5 succinimidyl ester; 2,5-Dioxopyrrolidin-1-yl 1-(5-{2-[(1E,3E)-5-{2-[2-(2,5-dioxopyrrolidin-1-yl)oxy]-2-oxoethyl}-3,3-dimethyl-5-sulfo-1-(5-sulfopentyl)indolin-2-ylidene]ethylidene}-2-[(1E,3E)-3,3-dimethyl-5-sulfo-1-(5-sulfopentyl)indolin-2-ylidene]cyclopent-1-en-1-yl)vinyl)-3,3-dimethyl-5-sulfo-3H-indol-1-ium-1-yl)pentane-1-sulfonate; Lumiprobe Sulfo-Cy5; Water-soluble Cy5 NHS ester
EINECS	Contact for details

Quality Control

Our Sulfo-Cy5 NHS Methyl Type is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for life science research. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 cm⁻¹M⁻¹
Solubility	Soluble in water, DMSO, DMF
Single Isomer Content (HPLC)	≥ 90.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.