Description

Tetramethylrhodamine Phalloidin is a high-purity, fluorescently labeled derivative of the potent toxin phalloidin, designed for selective and stable staining of filamentous actin (F-actin) in fixed cells. This compound is critical for researchers requiring precise visualization of the cytoskeleton in fluorescence microscopy applications, offering superior photostability and a bright red emission. It is an essential tool for cell biologists, neuroscientists, and pharmaceutical researchers in life science R&D, diagnostic assay development, and high-content screening.

Application

• Fluorescence Microscopy: Primary tool for visualizing and quantifying F-actin distribution, organization, and dynamics in fixed cell preparations.
• Cytoskeleton Research: Used in studies of cell morphology, motility, adhesion, and division by specifically staining the actin filament network.
• Neuroscience Studies: Labels actin structures in neurons, including growth cones, dendritic spines, and synaptic terminals.
• High-Content Screening (HCS): Employed in automated imaging platforms for phenotypic screening in drug discovery and toxicology.
• Diagnostic & Pathology: Can be used as a component in specialized staining kits for cellular analysis in research pathology.
• Multiplex Imaging: Ideal for co-staining experiments with other fluorescent probes (e.g., DAPI for nuclei, FITC-labeled antibodies) due to its distinct red emission.

Basic Information

Product Name	Tetramethylrhodamine Phalloidin
CAS No.	915013-10-4
Molecular Formula	C62H79N13O15S2 (Representative)
Molecular Weight	~1300 g/mol (Approx.)
Synonyms	TRITC-Phalloidin; Tetramethylrhodamine Isothiocyanate Phalloidin; Rhodamine Phalloidin; Phalloidin–Tetramethylrhodamine conjugate; Phalloidin-TRITC; F-Actin Probe, Tetramethylrhodamine Labeled; Actin-Tracker Red
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine Phalloidin is produced and handled under strict quality protocols to ensure batch-to-batch consistency and performance reliability for critical research. Each lot is characterized by advanced analytical techniques, including HPLC for purity and fluorescence spectroscopy for labeling efficiency. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Solutions should be prepared in appropriate buffers (e.g., methanol or PBS with BSA) and stored frozen in aliquots to avoid repeated freeze-thaw cycles. Allow the product to equilibrate to room temperature before opening.

Specification

Item	Specification
Appearance	Red to purple solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Extinction Coefficient (ε)	~95,000 cm⁻¹M⁻¹ at 540-550 nm
Emission Maximum	~575 nm
Excitation Maximum	~540 nm
Solubility	Soluble in methanol, DMSO, aqueous buffers
Functional Staining	Tested on control cell lines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.