Description

Atto 390 Acid CAS NO 914203-49-9 is a high-purity, fluorescent labeling reagent belonging to the Atto dye family, characterized by its carboxylic acid functional group. This compound is essential for creating stable, covalent conjugates with biomolecules such as proteins, antibodies, and oligonucleotides, enabling highly sensitive detection in advanced analytical techniques. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for applications requiring bright, photostable fluorescence in the near-UV to blue spectral region.

Application

• Biomolecule Labeling: Covalent conjugation to amines via its carboxylic acid group for labeling antibodies, proteins, peptides, and other biomolecules.
• Fluorescence Microscopy: Used as a bright and photostable fluorescent tag for cellular imaging, super-resolution microscopy (e.g., STED, PALM), and tracking studies.
• Flow Cytometry: Employed in the development of fluorescent probes and antibody conjugates for high-throughput cell analysis and sorting.
• Diagnostic Assays: Integration into lateral flow assays, ELISA, and other immunoassays as a detection label.
• Nucleic Acid Labeling: Conjugation to oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Sensor Development: A key component in the design of fluorescent chemical sensors and probes for detecting specific ions or small molecules.
• Material Science: Incorporation into polymers or nanoparticles to create functional fluorescent materials.

Basic Information

Product Name	Atto 390 Acid
CAS No.	914203-49-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ATTO 390 carboxylic acid; ATTO390-COOH; 2,5-Dihydro-2,2,5,5-tetramethylpyrrolo[3,4-c]pyrrole-1,4-dione, 6-(6-amino-3-imino-3H-xanthen-9-yl)-3,3-bis(2-carboxyethyl)-; ATTO 390 dye acid form; Fluorescent dye ATTO 390 acid; ATTO 390 SE precursor
EINECS	Contact for details

Quality Control

Every batch of Atto 390 Acid is produced and tested under stringent quality management systems. We ensure high lot-to-lot consistency through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry to verify identity, purity, and fluorescence performance. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting critical specifications. Our quality commitment supports applications in regulated environments, and custom purity grades or specific impurity profiles can be discussed to meet your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	390 nm ± 5 nm
Emission Maximum	Contact for details
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.