Description

n-(2-Azidoethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide is a sophisticated biotin derivative, engineered for advanced bioconjugation and click chemistry applications. This compound is critical for researchers and manufacturers requiring precise, covalent attachment of biotin tags to biomolecules, enabling sensitive detection and purification workflows. It is an essential intermediate for the pharmaceutical, diagnostic, and life science research sectors, where reliable, high-purity building blocks are paramount for developing novel therapeutics and assays.

Application

• Bioconjugation Reagent: Used as a key intermediate for synthesizing biotinylated probes, antibodies, and other biomolecules.
• Click Chemistry Substrate: The azidoethyl moiety enables efficient, bioorthogonal copper-catalyzed azide-alkyne cycloaddition (CuAAC) for labeling.
• Diagnostic Kit Development: Integral for creating high-sensitivity ELISA, lateral flow assays, and other diagnostic tools utilizing streptavidin-biotin technology.
• Affinity Purification Ligands: Serves in the synthesis of ligands for immobilization on solid supports to capture specific proteins or nucleic acids.
• Pharmaceutical Research: Employed in the discovery and development of targeted drug delivery systems and radiopharmaceuticals.
• Proteomics & Genomics: Facilitates the study of protein-protein interactions, post-translational modifications, and nucleic acid labeling.

Basic Information

Product Name	n-(2-Azidoethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide
CAS No.	912849-55-9
Molecular Formula	C14H21N7O2S
Molecular Weight	351.43 g/mol
Synonyms	5-[(3aS,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-(2-azidoethyl)pentanamide; Biotin-azide; Biotin-PEG3-Azide (common building block analog); N-(2-Azidoethyl)biotinamide; Azido-PEG3-biotin (related compound); Biotin azidoethylamide; Biotinylation reagent with azide handle.
EINECS	Contact for details

Quality Control

Our n-(2-Azidoethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for research and commercial applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers' regulatory and compliance needs in sensitive industries.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under inert atmosphere for long-term stability. This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.