Description

Cy5.5 NHS Ester is a near-infrared fluorescent labeling reagent widely used for the covalent conjugation of biomolecules. This compound is critical for creating stable, high-sensitivity probes for advanced imaging and diagnostic applications. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, in vivo imaging, flow cytometry, and immunoassay development.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of antibodies, proteins, peptides, and oligonucleotides for fluorescence-based assays.
• In Vivo Imaging: Development of near-infrared (NIR) fluorescent probes for deep-tissue imaging in preclinical research.
• Flow Cytometry & Microscopy: Creation of highly sensitive detection reagents for cellular analysis and imaging.
• Immunoassay Development: Labeling of detection antibodies for ELISA, Western blotting, and other immunoassay platforms.
• Diagnostic Reagent Manufacturing: Key component in the production of fluorescent diagnostic kits and reagents.
• Drug Delivery & Targeting Studies: Conjugation to nanoparticles or therapeutic agents to track biodistribution and targeting efficacy.

Basic Information

Product Name	Cy5.5 NHS Ester
CAS No.	910482-46-1
Molecular Formula	C46H51KN4O11S2
Molecular Weight	947.21 g/mol
Synonyms	Cy5.5 N-Hydroxysuccinimide Ester; Sulfo-Cyanine5.5 NHS Ester; Cy5.5 Succinimidyl Ester; 1-(5-Carboxypentyl)-1'-methyl-3,3,3',3'-tetramethyl-4,5,4',5'-dibenzo-2,2'-indotricarbocyanine N-hydroxysuccinimide ester potassium salt; NIR-783 NHS Ester; Cy™5.5 NHS Ester
EINECS	Contact for details

Quality Control

Our Cy5.5 NHS Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and labeling efficiency. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	≥ 190,000 cm⁻¹M⁻¹ (at λmax ~ 683 nm)
Fluorescence Emission (λmax)	~ 707 nm
Water Content (KF)	≤ 5.0%
Single Spot (TLC)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.