Description

n-(Dexamethasone 21-S-Ethyl-2'-Amino)Tetramethylrhodamine CAS NO 99143-17-6 is a high-purity, fluorescently labeled derivative of the potent glucocorticoid dexamethasone, designed for advanced research and diagnostic applications. This compound is critical for scientists requiring precise, high-sensitivity detection and tracking of glucocorticoid receptor activity and localization in complex biological systems. It is primarily utilized by researchers and developers in the fields of molecular biology, pharmacology, and high-content screening for drug discovery.

Application

• Fluorescence Microscopy & Imaging: Used as a vital probe for visualizing and tracking glucocorticoid receptors (GR) in live or fixed cells.
• High-Content Screening (HCS): Serves as a key reagent in automated screening assays to identify modulators of GR signaling pathways.
• Flow Cytometry: Enables the quantification and analysis of GR expression and internalization at the single-cell level.
• Biochemical Assay Development: Acts as a fluorescent tracer in competitive binding assays and studies of receptor-ligand interactions.
• Pharmacokinetic & Biodistribution Studies: Facilitates the study of drug delivery and tissue distribution of dexamethasone analogs in preclinical research.
• Diagnostic Probe Development: A potential component in the development of diagnostic kits for conditions related to glucocorticoid pathway dysregulation.

Basic Information

Product Name	n-(Dexamethasone 21-S-Ethyl-2'-Amino)Tetramethylrhodamine
CAS No.	99143-17-6
Molecular Formula	C52H61N3O7
Molecular Weight	840.06 g/mol
Synonyms	Dexamethasone-TAMRA Conjugate; Dexamethasone 21-(N-ethylamino)tetramethylrhodamine; 21-Aminoethyl-dexamethasone-TAMRA; TAMRA-Dexamethasone; Fluorescent Dexamethasone Derivative; Dexamethasone Rhodamine B Conjugate; Dexamethasone labeled with Tetramethylrhodamine; GR Fluorescent Ligand
EINECS	Contact for details

Quality Control

This high-grade biochemical is manufactured under strict quality protocols. Each batch is characterized and tested to ensure high purity and consistent performance in sensitive applications. Quality is verified via multiple analytical techniques including HPLC, mass spectrometry, and NMR. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. For long-term storage, keep under inert atmosphere.

Specification

Item	Specification
Appearance	Red to dark red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Extinction Coefficient	~92,000 cm-1M-1 (at 555 nm)
Fluorescence Emission	~580 nm
Solubility	Soluble in DMSO, DMF, methanol
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.