Description

5(6)-Carboxytetramethylrhodamine is a high-purity, reactive fluorescent dye derivative widely used as a critical labeling reagent. Its core value lies in its excellent photostability, high quantum yield, and the presence of a carboxylic acid group, which facilitates covalent conjugation to biomolecules. This compound is essential for researchers and manufacturers in the life sciences and diagnostics sectors who require reliable, high-performance fluorescent probes for advanced applications.

Application

• Biomolecule Labeling: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides via its carboxylic acid group for fluorescence-based assays.
• Fluorescent Probe Synthesis: A key intermediate in the preparation of advanced fluorescent probes, sensors, and tags for research and diagnostic kits.
• Immunofluorescence & Microscopy: Used to label primary or secondary antibodies for high-resolution cellular and tissue imaging (e.g., confocal, super-resolution microscopy).
• Flow Cytometry: Employed in the development of antibody conjugates for cell surface marker detection and analysis.
• Nucleic Acid Labeling: Conjugation to nucleotides or oligonucleotides for applications in fluorescence in situ hybridization (FISH) and real-time PCR.
• Diagnostic Assay Development: A critical component in the manufacturing of lateral flow assays, ELISA kits, and other point-of-care diagnostic devices.

Basic Information

Product Name	5(6)-Carboxytetramethylrhodamine
CAS No.	98181-63-6
Molecular Formula	C25H21N2O5
Molecular Weight	429.45 g/mol
Synonyms	5(6)-TAMRA; 5(6)-Carboxy-tetramethylrhodamine; Tetramethylrhodamine-5(6)-isomer carboxylic acid; 5(6)-Carboxy-TMR; 5- and 6-TAMRA, mixed isomers; 9-(2-Carboxyphenyl)-3,6-bis(dimethylamino)xanthylium chloride; TAMRA carboxylic acid; Rhodamine B isomeric carboxylate
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxytetramethylrhodamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, isomer ratio, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Total of 5- and 6- isomers)
Single Largest Unknown Impurity (HPLC)	≤ 1.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.