Description

2-((4-Azido-2-Nitrophenyl)Amino)Ethyl Triphosphate is a specialized nucleotide analog featuring a photoreactive azido group and a triphosphate moiety. This compound is critical for biochemical research and development, particularly in the study of nucleic acid interactions and enzyme mechanisms. It is primarily utilized by researchers in pharmaceutical R&D, molecular biology, and biochemistry for photoaffinity labeling, cross-linking studies, and the development of novel probes and inhibitors.

Application

• Photoaffinity Labeling Probe: Used to covalently tag and identify nucleic acid-binding proteins and enzymes upon UV light activation.
• Enzyme Mechanism Studies: Serves as a substrate analog for investigating the kinetics and active sites of polymerases, kinases, and other ATP-utilizing enzymes.
• Cross-linking Agent: Enables the study of protein-nucleic acid complexes and macromolecular interactions in structural biology.
• Bioconjugation & Probe Synthesis: A key building block for creating advanced diagnostic and research tools, such as fluorescent or affinity-tagged nucleotide derivatives.
• Drug Discovery Research: Employed in high-throughput screening assays to discover and characterize new therapeutic agents targeting nucleotide-binding sites.

Basic Information

Item	Details
Product Name	2-((4-Azido-2-Nitrophenyl)Amino)Ethyl Triphosphate
CAS No.	97467-06-6
Molecular Formula	C14H17N6O13P3
Molecular Weight	594.22 g/mol
Synonyms	2-((4-Azido-2-nitrophenyl)amino)ethyl triphosphate; ANP-ethyl triphosphate; 4-Azido-2-nitrophenylaminoethyl triphosphate; N-[2-(Triphosphonooxy)ethyl]-4-azido-2-nitroaniline; Photoreactive ATP analog; Azidophenyl nitroaniline ethyl triphosphate
EINECS	Contact for details

Quality Control

Our 2-((4-Azido-2-Nitrophenyl)Amino)Ethyl Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, NMR structural confirmation, and functional activity validation. Certificates of Analysis (COA) with detailed specifications are provided to guarantee the product meets the high standards required for biochemical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Tested in enzymatic assay (Contact for details)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.