Description

n-Succinimidyl-3-(2-Nitrovinyl)Benzoate is a specialized heterobifunctional crosslinking reagent featuring a photoreactive nitrovinyl group and an amine-reactive N-hydroxysuccinimide (NHS) ester. This compound is engineered for the efficient, site-specific conjugation of biomolecules, enabling the formation of stable amide bonds under mild conditions. It is an essential tool for researchers and manufacturers in bioconjugation, proteomics, and the development of diagnostic assays and targeted therapeutics, where precise and stable molecular linkages are critical.

Application

• Bioconjugation and Protein Labeling: Used to covalently attach proteins, peptides, or antibodies to various surfaces, particles, or other biomolecules via their primary amines.
• Photoreactive Crosslinking Agent: The nitrovinyl group allows for light-induced activation, enabling spatial and temporal control over the crosslinking process for studying protein-protein interactions.
• Diagnostic Kit Development: Employed in the synthesis of enzyme-antibody or hapten-protein conjugates for ELISA, lateral flow assays, and other immunoassay platforms.
• Polymer and Biomaterial Modification: Functionalizes polymers, hydrogels, or nanomaterials with bioactive ligands to create smart materials for drug delivery and tissue engineering.
• Affinity Chromatography Ligand Immobilization: Facilitates the stable coupling of antibodies, antigens, or other affinity ligands to chromatographic resins and solid supports.
• Probe Synthesis for Cellular Imaging: Serves as a key reagent in synthesizing fluorescent or other detectable probes for tracking biomolecules within cells.

Basic Information

Product Name	n-Succinimidyl-3-(2-Nitrovinyl)Benzoate
CAS No.	96441-29-1
Molecular Formula	C13H10N2O6
Molecular Weight	290.23 g/mol
Synonyms	3-(2-Nitrovinyl)benzoic acid N-succinimidyl ester; N-Succinimidyl 3-(2-nitrovinyl)benzoate; NHS ester of 3-(2-Nitrovinyl)benzoic acid; S-3-(2-Nitrovinyl)BA NHS ester; 3-(2-Nitroethenyl)benzoic acid 2,5-dioxopyrrolidin-1-yl ester; Heterobifunctional crosslinker, nitrovinyl NHS ester; Photoreactive NHS ester
EINECS	Contact for details

Quality Control

Our n-Succinimidyl-3-(2-Nitrovinyl)Benzoate is manufactured under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and moisture content analysis. We provide Certificates of Analysis (COA) with each shipment, detailing all specifications and test results to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.