Description

Fura-2 Ethyl Ester is a cell-permeable, fluorescent calcium indicator widely used in biological research. This compound is crucial for the non-invasive measurement of intracellular calcium ion (Ca²⁺) concentrations in live cells. It is an essential tool for researchers and professionals in neuroscience, pharmacology, and cell biology studying calcium signaling pathways, cellular physiology, and drug discovery.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic Ca²⁺ transients in live cells.
• Neuroscience Research: Monitoring neuronal activity, synaptic transmission, and calcium dynamics in neurons and glial cells.
• Cardiovascular Studies: Investigating calcium handling in cardiomyocytes, smooth muscle cells, and endothelial cells.
• Drug Discovery & Toxicology: Screening compounds for their effects on calcium signaling pathways and assessing cellular toxicity.
• Cell Physiology: Studying calcium's role in processes like muscle contraction, secretion, gene expression, and apoptosis.
• Fluorescence Microscopy & Flow Cytometry: Serving as a key fluorescent probe in high-content screening and cell population analysis.

Basic Information

Product Name	Fura-2 Ethyl Ester
CAS No.	96331-97-4
Molecular Formula	C₂₉H₂₈N₂O₁₃
Molecular Weight	612.54 g/mol
Synonyms	Fura-2 AM; Fura-2 Acetoxymethyl Ester; Fura-2, AM; Fura-2, acetoxymethyl ester; 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxyl]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester; 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid, acetoxymethyl ester; AM ester of Fura-2
EINECS	Contact for details

Quality Control

Our Fura-2 Ethyl Ester is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). For long-term stability, store in a freezer. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange-yellow solid or film
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Functional Activity	Effective loading and calcium response in cell-based assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.