Description

Fura 2 is a high-purity, cell-permeant acetoxymethyl (AM) ester derivative widely used as a fluorescent calcium indicator in biological research. Its core value lies in its ability to selectively chelate intracellular calcium ions, producing a significant and quantifiable shift in fluorescence emission, which is critical for real-time monitoring of cellular calcium dynamics. This compound is essential for researchers and professionals in neuroscience, pharmacology, and cell biology who require precise tools for studying calcium signaling pathways, receptor function, and cellular physiology.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization and quantification of cytosolic free Ca²⁺ concentrations in live cells.
• Neuroscience Research: Used to study calcium flux in neurons, synaptic transmission, and activity-dependent processes.
• Cardiovascular Research: Monitoring calcium transients in cardiac myocytes and vascular smooth muscle cells.
• Drug Discovery & Screening: Employed in high-throughput assays to evaluate the effects of pharmacological agents on calcium mobilization.
• Cell Signaling Studies: Investigating G-protein coupled receptor (GPCR) activation, ion channel function, and second messenger systems.
• Fluorescence Microscopy & Flow Cytometry: A key reagent for single-cell analysis and population studies using fluorescence-based detection systems.

Basic Information

Product Name	Fura 2
CAS No.	96314-98-6
Molecular Formula	C₂₉H₃₀N₂O₁₈
Molecular Weight	694.55 g/mol
Synonyms	Fura-2 AM; Fura-2 acetoxymethyl ester; Fura-2, AM ester; Fura 2, pentapotassium salt; 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxyl]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxymethyl ester; Fura-2, pentapotassium; Calcium indicator Fura-2 AM; BAPTA derivative Fura-2
EINECS	Contact for details

Quality Control

Our Fura 2 is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral analysis for correct fluorescence properties, and functional validation in cell-based assays. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Properties	Conforms to reference
Functional Activity (Cell Assay)	Pass

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.