Description

Indo 1 Pentapotassium Salt is a high-purity, cell-permeant fluorescent calcium indicator salt widely used in biological research. Its core value lies in its high affinity for calcium ions, enabling precise quantitative measurement of intracellular calcium concentration ([Ca²⁺]i) dynamics. This product is essential for researchers and professionals in neuroscience, cardiology, pharmacology, and cell biology who require reliable tools for studying cellular signaling pathways, ion channel function, and drug discovery.

Application

• Intracellular Calcium Imaging: Primary use for real-time visualization and quantification of cytosolic calcium transients in live cells using fluorescence microscopy (e.g., confocal, epifluorescence).
• Neuroscience Research: Monitoring neuronal activity, synaptic transmission, and calcium signaling in neurons and glial cells.
• Cardiovascular Studies: Investigating calcium handling, excitation-contraction coupling, and arrhythmogenic mechanisms in cardiomyocytes.
• Drug Screening & Toxicology: Assessing the effects of pharmaceutical compounds, toxins, or environmental stressors on cellular calcium homeostasis.
• Cell Physiology & Signal Transduction: Studying GPCR (G-protein coupled receptor) activation, store-operated calcium entry (SOCE), and other signaling cascades.
• High-Throughput Screening (HTS): Compatible with plate reader assays for automated, large-scale pharmacological or genetic screens.

Basic Information

Product Name	Indo 1 Pentapotassium Salt
CAS No.	96314-96-4
Molecular Formula	C47H49K5N2O23S2
Molecular Weight	1260.55 g/mol
Synonyms	Indo-1, Pentapotassium Salt; Indo-1 K+ Salt; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentapotassium salt; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9H-xanthen-9-yl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentapotassium salt; Calcium indicator Indo-1; Indo-1, cell permeant; AM ester derivative precursor.
EINECS	Contact for details

Quality Control

Our Indo 1 Pentapotassium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence characteristics, and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting the product's compliance with our stringent in-house specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will degrade product performance. Aliquot contents upon receipt to minimize freeze-thaw cycles and maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange-yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Conforms to reference (λex ~355 nm, λem ratio ~405/485 nm with Ca²⁺)
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.