Description

n-(4-Azido-2-Nitrophenyl)-2-Aminoethyl Diphosphate is a specialized biochemical reagent featuring a photoreactive azido group and a diphosphate moiety. This compound matters as a critical tool for photoaffinity labeling and cross-linking studies, enabling researchers to probe protein-ligand interactions and enzyme active sites with high specificity. It is primarily needed by research scientists and process developers in the fields of biochemistry, enzymology, and pharmaceutical research for mechanistic studies and probe development.

Application

• Photoaffinity Labeling Probe: Used to covalently tag and identify protein binding sites upon UV light activation.
• Enzyme Mechanism Studies: Serves as an active site-directed probe for kinases, ATPases, and other nucleotide-binding enzymes.
• Bioconjugation & Cross-linking: Facilitates the creation of stable conjugates between biomolecules for analytical or therapeutic purposes.
• Biochemical Research Reagent: Employed in academic and industrial R&D for investigating nucleotide-dependent processes.
• Proteomics & Target Identification: Aids in the discovery and validation of molecular targets in drug discovery pipelines.
• Diagnostic Kit Development: Can be utilized in the development of sensitive assay systems for clinical diagnostics.

Basic Information

Product Name	n-(4-Azido-2-Nitrophenyl)-2-Aminoethyl Diphosphate
CAS No.	96189-14-9
Molecular Formula	C8H11N4O10P2
Molecular Weight	400.14 g/mol
Synonyms	2-Aminoethyl (4-Azido-2-nitrophenyl) Diphosphate; 4-Azido-2-nitrophenylaminoethyl Diphosphate; ANP-Diphosphate; Photoactivatable ATP Analog; N-(4-Azido-2-nitrophenyl)-2-aminoethyl Diphosphoric Acid Ester; 2-[(4-Azido-2-nitrophenyl)amino]ethyl Diphosphate; 96189-14-9
EINECS	Contact for details

Quality Control

Our production of n-(4-Azido-2-Nitrophenyl)-2-Aminoethyl Diphosphate adheres to stringent in-process controls to ensure batch-to-batch consistency and high purity, suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. Allow the container to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or solution
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.