Description

Bis(Choline)-n-(4-Nitrobenzo-2-Oxa-1,3-Diazol-7-Yl)Imino Dipropionate is a specialized choline derivative functionalized with a nitrobenzoxadiazole (NBD) fluorophore, designed for advanced research and diagnostic applications. This compound matters because it serves as a critical tool for labeling and tracking choline metabolism and transport in biological systems with high sensitivity. Researchers in pharmaceutical development, neurochemistry, and molecular biology need it for creating fluorescent probes to study cellular processes, enzyme activity, and receptor interactions in real-time.

Application

• Fluorescent Labeling Agent: Used to tag choline, acetylcholine, or related molecules for fluorescence microscopy and imaging studies.
• Biochemical Research Probe: Serves as a substrate or inhibitor in enzymatic assays to study choline kinases, acetyltransferases, and transporters.
• Neurochemical Tracer: Applied in neuroscience research to visualize and quantify cholinergic neuron activity and synaptic function.
• Diagnostic Kit Component: Incorporated into in-vitro diagnostic (IVD) kits for the detection of choline-related metabolic disorders.
• Cell Biology Studies: Utilized for tracking phospholipid synthesis and cell membrane dynamics in live-cell assays.
• Drug Discovery Tool: Employed in high-throughput screening (HTS) to identify modulators of cholinergic pathways.

Basic Information

Product Name	Bis(Choline)-n-(4-Nitrobenzo-2-Oxa-1,3-Diazol-7-Yl)Imino Dipropionate
CAS No.	93775-36-1
Molecular Formula	C₂₀H₃₂N₆O₈
Molecular Weight	484.51 g/mol
Synonyms	NBD-Choline Dipropionate; 7-[(2,2-Bis(cholineoxycarbonyl)ethyl]amino]-4-nitrobenz-2-oxa-1,3-diazole; Bis(choline) NBD-iminodipropionate; NBD-C2-Choline; Fluorescent Choline Analog; Choline-NBD Conjugate; 4-Nitro-7-[(2,2-bis(2-hydroxyethyl)dimethylammonio)ethoxycarbonyl)ethyl]amino]-2,1,3-benzoxadiazole
EINECS	Contact for details

Quality Control

Our Bis(Choline)-n-(4-Nitrobenzo-2-Oxa-1,3-Diazol-7-Yl)Imino Dipropionate is produced under strict quality management systems to ensure batch-to-batch consistency for research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and fluorometric analysis to verify performance. Comprehensive Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the high standards required for sensitive biochemical and cellular studies.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is light-sensitive (store away from light) and hygroscopic. For long-term stability, keep the container under an inert atmosphere such as argon or nitrogen. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Fluorescence Properties	Ex/Em ~465/535 nm in buffer
Solubility	Soluble in DMSO, Methanol, Water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.