Description

m-Maleimidobenzoyl-n-Hydroxysulfosuccinimide Ester is a heterobifunctional crosslinking reagent featuring a maleimide group and an amine-reactive NHS ester, with the latter being sulfonated for enhanced water solubility. This compound is critical for creating stable, site-specific conjugates between thiol-containing molecules (like proteins or peptides) and amine-containing targets. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and advanced diagnostics.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key reagent for constructing stable, cleavable, or non-cleavable linkers between cytotoxic drugs and monoclonal antibodies.
• Protein-Protein/Protein-Peptide Conjugation: Enables the site-specific crosslinking of thiolated proteins to amine-containing partners for structural studies or therapeutic protein engineering.
• Fluorescent Probe & Diagnostic Reagent Labeling: Used to attach dyes, enzymes (HRP, AP), or other reporter molecules to antibodies, peptides, or oligonucleotides for immunoassays and imaging.
• Surface Immobilization & Bioconjugation: Functionalizes gold nanoparticles, chips, or beads with proteins or peptides for biosensor development and affinity chromatography.
• Polymer & Biomaterial Modification: Incorporates bioactive ligands into hydrogels, polymer backbones, or drug delivery vehicles to create smart, responsive materials.
• Enzyme & Catalyst Immobilization: Facilitates the stable attachment of enzymes to solid supports while aiming to preserve catalytic activity.

Basic Information

Product Name	m-Maleimidobenzoyl-n-Hydroxysulfosuccinimide Ester
CAS No.	92921-25-0
Molecular Formula	C15H12N2O9S
Molecular Weight	396.33 g/mol
Synonyms	Sulfo-MBS; m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester; 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid N-hydroxysulfosuccinimide ester sodium salt (common mis-assignment); 3-Maleimidobenzoic acid N-hydroxysulfosuccinimide ester; Sulfo-MBS crosslinker; m-Maleimidobenzoyl sulfosuccinimidyl ester; Water-soluble MBS; Heterobifunctional crosslinker sulfo-MBS; Sodium 4-((2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)methyl)cyclohexane-1-carboxylate-2,5-dioxopyrrolidin-1-yl ester sulfonate (related structure)
EINECS	Contact for details

Quality Control

Our m-Maleimidobenzoyl-n-Hydroxysulfosuccinimide Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity, solubility, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Freely soluble in water, DMSO
Functional Activity	Conjugation efficiency ≥ 90% (by control test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.