Description

5-Carboxyfluorescein N-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in biochemistry and molecular biology. Its core value lies in its ability to form stable amide bonds with primary amines, facilitating the sensitive detection and quantification of biomolecules. This compound is essential for researchers and manufacturers in the fields of bioconjugation, immunoassay development, and flow cytometry, where reliable fluorescent tagging is critical.

Application

• Protein and Antibody Labeling: Conjugation to lysine residues for fluorescence-activated cell sorting (FACS) and microscopy.
• Oligonucleotide Probes: Tagging of amino-modified DNA or RNA for in situ hybridization and real-time PCR.
• Biomolecule Tracking: Fluorescent labeling of peptides, enzymes, and other amine-containing compounds for kinetic and localization studies.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent immunoassays and biosensors.
• Cell Biology Research: Intracellular labeling and tracing applications in live and fixed cells.
• Material Science: Surface functionalization of nanoparticles and polymers for bioimaging and targeted delivery systems.

Basic Information

Product Name	5-Carboxyfluorescein N-Succinimidyl Ester
CAS No.	92557-80-7
Molecular Formula	C25H15NO9
Molecular Weight	473.39 g/mol
Synonyms	5(6)-FAM, SE; 5-Carboxyfluorescein Succinimidyl Ester; 6-Carboxyfluorescein N-Hydroxysuccinimide Ester; CFSE; FAM, SE; Fluorescein-5(6)-Carboxamidocaproic Acid N-Succinimidyl Ester; 5(6)-Carboxyfluorescein N-Succinimidyl Ester
EINECS	Contact for details

Quality Control

Our 5-Carboxyfluorescein N-Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Absorbance (Max)	492 nm ± 2 nm
Emission (Max)	518 nm ± 2 nm
Extinction Coefficient	~83,000 cm-1M-1
Solubility	DMSO, DMF
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.