Description

4',5'-Dimethylfluorescein Isothiocyanate is a chemically reactive derivative of fluorescein, specifically designed for labeling and detection applications. This compound matters for its enhanced photostability and specific reactivity, making it a superior choice for creating stable, fluorescent conjugates. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors for applications such as fluorescence microscopy, flow cytometry, and immunoassay development.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for detection and imaging.
• Cell Staining and Tracking: Used in flow cytometry and fluorescence microscopy for intracellular labeling and cell population analysis.
• Diagnostic Assay Development: A key component in the manufacture of fluorescent probes and kits for clinical diagnostics and research immunoassays (ELISA, Western Blot).
• Bioconjugation Research: Serves as a critical reagent in developing novel bio-conjugates and studying biomolecular interactions.
• Material Science Markers: Potential use in tagging polymers or nanoparticles for tracking and characterization in advanced materials research.

Basic Information

Product Name	4',5'-Dimethylfluorescein Isothiocyanate
CAS No.	92457-52-8
Molecular Formula	C25H17NO6S
Molecular Weight	459.47 g/mol
Synonyms	5(6)-FITC, 4',5'-Dimethyl isomer; 4',5'-Dimethylfluorescein 5(6)-isothiocyanate; 4',5'-Dimethyl-FITC; Fluorescein isothiocyanate, 4',5'-dimethyl-; 5-Isothiocyanato-4',5'-dimethylfluorescein; 6-Isothiocyanato-4',5'-dimethylfluorescein; FITC, 4',5'-Dimethyl derivative
EINECS	Contact for details

Quality Control

Our 4',5'-Dimethylfluorescein Isothiocyanate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below. This product is hygroscopic (moisture-sensitive) and strictly light-sensitive; protect from all light exposure and moisture to maintain stability and performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~498 nm (in buffer)
Emission Maximum	~518 nm (in buffer)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer
Moisture (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.