Description

5-Carboxytetramethylrhodamine is a high-purity, reactive derivative of the widely used tetramethylrhodamine (TAMRA) fluorophore. This compound is essential for creating bright, photostable fluorescent conjugates, particularly for advanced bioanalytical and diagnostic applications. It is primarily needed by manufacturers and researchers in the life sciences, diagnostics, and biotechnology sectors for labeling proteins, antibodies, and oligonucleotides.

Application

• Fluorescent Labeling Reagent: A key intermediate for synthesizing amine-reactive NHS esters and other active esters used to label proteins, peptides, and antibodies.
• Bioconjugation: Used in the preparation of fluorescent probes for flow cytometry, fluorescence microscopy (e.g., confocal), and immunofluorescence assays.
• Nucleic Acid Labeling: Employed in the synthesis of labeled nucleotides and oligonucleotide probes for fluorescence in situ hybridization (FISH) and real-time PCR.
• Diagnostic Assays: Serves as a critical fluorescent tag in the development of lateral flow assays, ELISA kits, and other clinical diagnostic tools.
• Sensor Development: Used in the construction of fluorescent sensors and indicators for detecting various biological analytes.
• Material Science: Can be incorporated into polymers and nanoparticles to create functional fluorescent materials for tracking and imaging.

Basic Information

Product Name	5-Carboxytetramethylrhodamine
CAS No.	91809-66-4
Molecular Formula	C25H21N2O5Cl
Molecular Weight	464.90 g/mol
Synonyms	5(6)-Carboxy-tetramethylrhodamine; 5(6)-TAMRA; 5-Carboxy-TAMRA; 5(6)-Carboxytetramethylrhodamine; Tetramethylrhodamine-5(6)-carboxylic acid; 5(6)-Carboxy-N,N,N',N'-tetramethylrhodamine; TAMRA-5(6)-carboxylic acid; Rhodamine derivative 5-carboxy
EINECS	Contact for details

Quality Control

Our 5-Carboxytetramethylrhodamine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); keep the container tightly sealed in a desiccated environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~540-550 nm
Emission Maximum	~565-575 nm
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.