Description

4'-(Aminomethyl)Fluoresceinehydrochloride is a chemically modified derivative of fluorescein, featuring an aminomethyl group that provides a reactive handle for conjugation. This compound is valued for its ability to serve as a versatile fluorescent labeling and detection reagent in advanced biochemical assays. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as probe synthesis and fluorescence-based detection.

Application

• Fluorescent Labeling Reagent: Used for covalently tagging biomolecules like proteins, peptides, and oligonucleotides for detection and imaging.
• Bioconjugation & Probe Synthesis: Serves as a key intermediate in the synthesis of fluorescent probes and sensors for biological targets.
• Diagnostic Assay Development: Employed in the development of fluorescence-based diagnostic kits and immunoassays.
• Cell Biology & Microscopy: Utilized for fluorescent staining and tracking in cellular and tissue imaging studies.
• Biochemical Research: Acts as a tool compound in enzyme activity assays and other fluorescence detection research methodologies.
• Pharmaceutical Analysis: Applied in drug discovery for high-throughput screening and molecular interaction studies.

Basic Information

Product Name	4'-(Aminomethyl)Fluoresceinehydrochloride
CAS No.	91539-64-9
Molecular Formula	C22H18ClNO5
Molecular Weight	411.83 g/mol
Synonyms	5-(Aminomethyl)fluorescein hydrochloride; Fluorescein-5-amine hydrochloride; 6-Aminomethylfluorescein hydrochloride; 4'-Aminomethylfluorescein HCl; 5-AMF HCl; FAM-amine hydrochloride; 5-(Aminomethyl)-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one hydrochloride
EINECS	Contact for details

Quality Control

Our 4'-(Aminomethyl)Fluoresceinehydrochloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) detailing batch-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, Methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.