Description

5,6-Dicarboxyfluorescein is a high-purity, functionalized derivative of fluorescein, a cornerstone fluorophore in life science research. Its value lies in the presence of two carboxylic acid groups, which provide reactive handles for covalent conjugation to biomolecules like proteins, peptides, and nucleic acids. This compound is essential for researchers and manufacturers in the fields of bioconjugation, fluorescent probe development, and diagnostic assay design, enabling the creation of sensitive detection systems.

Application

• Fluorescent Labeling Reagent: Primary use as a reactive dye for labeling antibodies, proteins, and other biomolecules via carbodiimide or other coupling chemistries.
• Bioconjugation Intermediate: Serves as a key building block for synthesizing custom fluorescent probes and tags with specific targeting properties.
• Diagnostic Assay Development: Used in the manufacture of reagents for fluorescence-based immunoassays (FLISA), lateral flow tests, and other clinical diagnostics.
• Molecular Biology Probes: Conjugation to oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Sensor and Imaging Agent Synthesis: A precursor for developing pH-sensitive fluorescent sensors and contrast agents for cellular imaging.
• Material Science: Incorporation into polymers or nanoparticles to create fluorescent materials for tracking and analysis.

Basic Information

Product Name	5,6-Dicarboxyfluorescein
CAS No.	90829-94-0
Molecular Formula	C24H14O9
Molecular Weight	446.36 g/mol
Synonyms	5(6)-Carboxyfluorescein; 5,6-Carboxyfluorescein; 5(6)-FAM; Fluorescein-5(6)-carboxylic acid; 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-5-(or 6-)carboxybenzoic acid; 5(6)-Carboxy-3',6'-dihydroxyfluoran; 5,6-FAM
EINECS	Contact for details

Quality Control

Our 5,6-Dicarboxyfluorescein is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and other critical parameters is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95% (Area %)
Absorption Max (λmax)	~492 nm
Emission Max (λem)	~518 nm
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.