Description

Erythrosin-5-Isothiocyanate is a reactive fluorescent dye derivative, widely recognized for its utility as a covalent labeling reagent in biochemical and biotechnological research. This compound is specifically engineered to form stable thiourea bonds with primary amines, enabling the precise tagging and detection of proteins, antibodies, and other biomolecules. It is an essential tool for researchers and manufacturers in the fields of immunoassay development, fluorescence microscopy, and flow cytometry, where high-sensitivity and specific conjugation are paramount.

Application

• Fluorescent Labeling of Proteins and Antibodies: Covalent conjugation for use in immunofluorescence (IF), Western blotting, and ELISA development.
• Cell and Tissue Staining: Used as a probe in fluorescence microscopy and histochemistry for visualizing cellular structures.
• Flow Cytometry Reagents: Conjugation to detection antibodies or streptavidin for multiparameter cell analysis.
• Biomolecular Interaction Studies: Labeling of ligands or receptors for fluorescence polarization (FP) or fluorescence resonance energy transfer (FRET) assays.
• Diagnostic Kit Manufacturing: Key component in the production of fluorescent lateral flow assays and other in-vitro diagnostic (IVD) devices.
• Polymer and Material Science: Modification of amine-functionalized surfaces or nanomaterials for sensor applications.

Basic Information

Product Name	Erythrosin-5-Isothiocyanate
CAS No.	90284-47-2
Molecular Formula	C23H8I4N2O5S
Molecular Weight	897.93 g/mol
Synonyms	Erythrosin B isothiocyanate; Erythrosin ITC; Acid Red 51 isothiocyanate; 2-(6-Hydroxy-2,4,5,7-tetraiodo-3-oxo-3H-xanthen-9-yl)benzoic acid isothiocyanate; 5-Isothiocyanatofluorescein tetraiodide; FD&C Red No. 3 isothiocyanate; C.I. 45430 isothiocyanate
EINECS	Contact for details

Quality Control

Our Erythrosin-5-Isothiocyanate is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency, and functional validation in conjugation assays. Certificates of Analysis (COA) with detailed specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after each use to maintain stability and reactivity.

Specification

Item	Specification
Appearance	Dark red to brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	528 - 534 nm
Extinction Coefficient (ε)	~84,000 cm-1M-1
Fluorescence Emission	~550 nm
Water Content (KF)	≤ 2.0%
Functional Activity (Labeling)	≥ 90% conjugation efficiency

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.