Description

Di-4-Anepps is a specialized organic compound widely recognized for its utility as a voltage-sensitive dye in biomedical and biophysical research. Its primary value lies in its ability to optically monitor changes in membrane potential, providing critical insights into cellular and neuronal activity. This makes it an essential reagent for researchers and manufacturers in the fields of neuroscience, cardiology, and pharmaceutical screening, where precise electrophysiological measurements are paramount.

Application

• Neuroscience Research: Used as a potentiometric dye for optical imaging of action potentials and synaptic activity in neurons and brain slices.
• Cardiac Electrophysiology: Employed to visualize and analyze transmembrane potential changes in cardiac myocytes and tissue for drug discovery and safety pharmacology.
• High-Throughput Screening (HTS): A key component in fluorescence-based assays for screening compounds that modulate ion channels and membrane potential in drug development.
• Cellular Physiology Studies: Facilitates the study of membrane dynamics, ion transport, and cellular excitability in various cell types.
• Diagnostic Reagent Formulation: Incorporated into specialized kits and reagents for research-grade diagnostic applications.

Basic Information

Product Name	Di-4-Anepps
CAS No.	90134-00-2
Molecular Formula	C29H31N2O6S2+
Molecular Weight	567.70 g/mol
Synonyms	Di-4-ANEPPS; 4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium, inner salt; JPW 1114; Voltage-sensitive dye JPW 1114; Di-4-ANEPPS (JPW 1114); Potential-sensitive dye Di-4-ANEPPS
EINECS	Contact for details

Quality Control

Our Di-4-Anepps is produced under stringent quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectroscopic methods for identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment. For long-term stability, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to black crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Absorption/Emission	Characteristic spectra provided in COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.