Description

Toluidine Blue O is a synthetic metachromatic thiazine dye with significant biological staining properties. This compound is valued for its selective affinity to specific tissue components, making it a critical tool in diagnostic and research histology. It is primarily utilized by professionals in the life sciences, medical diagnostics, and industrial quality control sectors for precise cellular and tissue analysis.

Application

• Histological Staining: Widely used as a metachromatic stain for mast cells, cartilage, and mucopolysaccharides in tissue sections for microscopic examination.
• Cytological Analysis: Employed in cytology for staining nucleic acids (DNA and RNA) and identifying cellular structures in smears and preparations.
• Microbiological Staining: Applied in microbiology for staining certain bacteria and fungi to enhance contrast and visibility under a microscope.
• Metachromasia Studies: A key reagent for demonstrating metachromasia in tissues, aiding in the diagnosis of certain metabolic storage diseases.
• Textile Industry: Used as a colorant for dyeing silk, wool, and nylon fibers.
• Ink Manufacturing: Serves as a component in the formulation of specialized inks and biological markers.
• Research & Development: Utilized in biochemical and pharmaceutical research for various staining protocols and experimental assays.

Basic Information

Product Name	Toluidine Blue O
CAS No.	92-31-9
Molecular Formula	C15H16ClN3S
Molecular Weight	305.82 g/mol
Synonyms	Toluidine Blue; Tolonium Chloride; Basic Blue 17; C.I. 52040; Toluidine Blue O; Toluidine Blue Zn Free; 3-Amino-7-(dimethylamino)-2-methylphenothiazin-5-ium chloride; TBO
EINECS	202-146-2

Quality Control

Our Toluidine Blue O is manufactured under a strict quality management system to ensure batch-to-batch consistency and reliability. Each lot is tested to confirm identity, purity, and performance in standard staining applications. A comprehensive Certificate of Analysis (COA) detailing specifications and test results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at a controlled room temperature (15-25°C). Keep away from incompatible materials. The product is light-sensitive and should be stored away from direct light exposure.

Specification

Item	Specification
Appearance	Dark green powder with metallic luster
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0%
Solubility (in Water)	Clear, blue solution
Heavy Metals (as Pb)	≤ 20 ppm
Staining Performance	Meets biological staining criteria

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.