Description

Atto 425-Nhs Ester is a high-purity, reactive fluorescent dye derivative designed for advanced bioconjugation applications. This compound is critical for researchers and manufacturers requiring stable, covalent labeling of biomolecules such as proteins, peptides, and antibodies with a bright, photostable blue-fluorescent tag. It is an essential tool for professionals in biotechnology, pharmaceutical development, and diagnostic assay manufacturing who need reliable and efficient fluorescent probes for imaging, detection, and analysis.

Application

• Bioconjugation and Protein Labeling: Covalent attachment to primary amines (-NH2) on proteins, antibodies, and peptides for fluorescence-activated cell sorting (FACS), immunofluorescence, and Western blotting.
• Biomolecular Imaging and Microscopy: Used as a fluorescent tracer in super-resolution microscopy (e.g., STED, PALM) and live-cell imaging studies due to its photostability.
• Diagnostic Assay Development: Labeling of capture antibodies or antigens in lateral flow assays (LFAs), enzyme-linked immunosorbent assays (ELISAs), and other in vitro diagnostic (IVD) kits.
• Nucleic Acid Labeling: Conjugation to amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR detection.
• Material Science and Sensor Development: Functionalization of nanoparticles, polymers, and surfaces to create fluorescent biosensors and smart materials.
• Drug Discovery and High-Throughput Screening (HTS): Tagging of target molecules or screening compounds in binding and inhibition assays.

Basic Information

Product Name	Atto 425-Nhs Ester
CAS No.	892156-28-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	ATTO 425 NHS ester; ATTO 425 N-hydroxysuccinimide ester; ATTO 425, SE; 5-Isomer ATTO 425 NHS ester; Succinimidyl ester of ATTO 425; Fluorescent dye NHS ester; Reactive blue fluorescent dye
EINECS	Contact for details

Quality Control

Our Atto 425-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Blue to dark blue solid or film
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~425 nm
Emission Maximum (λem)	~480 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.