Description

Fluorescent Red Mega 485 Nhs-Ester CAS NO 890317-36-9 is a high-performance, reactive fluorescent dye derivative designed for advanced bioconjugation and labeling applications. This compound is engineered to provide exceptional brightness and photostability, enabling highly sensitive detection and imaging in complex biological systems. It is an essential tool for researchers and manufacturers in the fields of life sciences, diagnostics, and pharmaceutical development who require reliable, high-quality fluorescent probes for protein, antibody, and nucleic acid labeling.

Application

• Bioconjugation and Protein Labeling: Covalently attaches to primary amines (e.g., lysine residues) on antibodies, proteins, and peptides for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and Western blotting.
• Diagnostic Assay Development: Used as a critical component in the manufacture of fluorescent immunoassays (FLIA), lateral flow tests, and other in vitro diagnostic (IVD) reagents.
• Bioimaging and Microscopy: Enables high-resolution cellular and tissue imaging in confocal, super-resolution, and live-cell microscopy due to its red emission and excellent signal-to-noise ratio.
• Nucleic Acid Detection: Can be conjugated to oligonucleotides or nucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Drug Discovery & High-Throughput Screening (HTS): Serves as a tracer or reporter molecule in target identification, binding assays, and cellular pathway analysis.
• Material Science & Sensor Fabrication: Incorporated into polymers, nanoparticles, and biosensors to create smart materials with optical reporting capabilities.

Basic Information

Item	Details
Product Name	Fluorescent Red Mega 485 Nhs-Ester
CAS No.	890317-36-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mega 485 NHS Ester; Fluorescent Red Mega 485 N-hydroxysuccinimide ester; Red Mega 485 SE; 485 Nhs Ester; Reactive Red Fluorescent Dye 485; N-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-N-ethyl-4-((E)-2-((E)-3-((E)-2-(1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)ethylidene)-2-((E)-2-(1-ethyl-3,3-dimethylindolin-2-ylidene)ethylidene)cyclopent-1-en-1-yl)vinyl)-1-methylquinolin-1-ium-6-sulfonate
EINECS	Contact for details

Quality Control

Every batch of Fluorescent Red Mega 485 Nhs-Ester is manufactured under a strict quality management system and undergoes comprehensive analytical testing. We ensure high purity and consistent performance through techniques including HPLC, NMR, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and functional performance is provided with each shipment to guarantee it meets the rigorous standards required for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~ 485 nm
Emission Maximum (λem)	~ 610 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF
Moisture Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.