Description

Rhod-5N (Potassium Salt) is a high-purity, cell-permeant fluorescent calcium indicator widely used in life science research. Its core value lies in its high affinity for calcium ions, enabling precise measurement of intracellular calcium dynamics in live cells. This compound is essential for researchers in neurobiology, cardiology, and cell signaling who require reliable and sensitive tools for fluorescence imaging and flow cytometry. The product is supplied as a high-quality potassium salt to ensure optimal solubility and performance in aqueous biological buffers.

Application

• Intracellular Calcium Imaging: Primary application for real-time visualization of calcium flux in neurons, cardiomyocytes, and other cell types using fluorescence microscopy.
• Flow Cytometry: Used to measure and sort cell populations based on their intracellular calcium levels.
• Drug Discovery & Screening: Employed in high-throughput assays to evaluate the effect of pharmacological compounds on calcium signaling pathways.
• Physiological Research: Critical for studying calcium's role in muscle contraction, neurotransmitter release, and apoptosis.
• Diagnostic Assay Development: Serves as a key reagent in developing in vitro diagnostic tests for conditions involving calcium dysregulation.

Basic Information

Product Name	Rhod-5N (Potassium Salt)
CAS No.	888229-31-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Rhod-5N, K+ salt; Rhod-5N Potassium Salt; Rhod-5N, pentapotassium salt; Calcium indicator Rhod-5N; Fluorescent calcium dye Rhod-5N; Rhod-5N AM ester (precursor form); X-Rhod-5N (related analog); Benzoxazolium fluorescent probe
EINECS	Contact for details

Quality Control

Our Rhod-5N (Potassium Salt) is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for critical research. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectrophotometric validation of its fluorescent properties. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming identity, purity, and suitability for fluorescence-based applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em: ~549/580 nm (Ca2+ bound); Conforms to reference spectrum
Solubility	Soluble in DMSO, water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.